SARS CoV-2 Spike protein nanodisc complex
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The SPIKE protein is the surface protien of SARS-CoV-2, more commonly known as the... more
Product information "SARS CoV-2 Spike protein nanodisc complex"
The SPIKE protein is the surface protien of SARS-CoV-2, more commonly known as the coronavirus. It interacts on the cell's surface with the serine protease TMPRSS2 and the surface receptor ACE2.
Native spike protein conists of a trimer that is based in the virus membrane. Therefore purifiy native & active SPIKE the trimer needs to be kept intact. Cube Biotech achieved this in three ways:
Native spike protein conists of a trimer that is based in the virus membrane. Therefore purifiy native & active SPIKE the trimer needs to be kept intact. Cube Biotech achieved this in three ways:
- MSP nanodiscs, as you can see here
- Detergent Mycelles
- Synthetic nanodiscs
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Figure 1: SDS Page of purified SPIKE protein in MSP1D1 nanodiscs. The band that represents the MSP protein can also be seen.
Figure 2: Gel filtration result of SPIKE in MSP nanodisc. The peak that represents spike is marked with a red arrow.Features
| Alternative names | SPIKE_SARS2 Spike glycoprotein |
| UniProt number | P0DTC2 |
| Protein class | Single span transmembrane protein |
| Organism | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| Sequence |
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| Affinity tag | C-terminal Rho1D4 |
| Expression Host | Hek293 Expi cells |
| Size | 1286 amino acids (including Rho1D4 tag and linker) 142114 Da |
| Buffer composition | 20 mM Hepes pH 7.5; 150 mM NaCl, |
| Nanodisc | MSP1D1-His, assembly with POPC lipids (Membrane scaffold protein based nanodisc; MSP1D1 = 25,3 kDa) |
| Function | host cell surface receptor binding; fusion of virus membrane with host endosome membrane |
| Purity (SDS-PAGE) | >98% as determined by SDS-PAGE, see Fig. 1 A and B |
| Homogeneity | >Size exclusion chromatography, Superose 6 Increase, see Fig. 2 |
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