SARS CoV-2 Spike protein nanodisc complex

€1,148.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

µG:

  • 29301
The SPIKE protein is the surface protien of SARS-CoV-2, more commonly known as the... more
Product information "SARS CoV-2 Spike protein nanodisc complex"
Product Type: Spike protein
Protein Sequence: Full-length
The SPIKE protein is the surface protien of SARS-CoV-2, more commonly known as the coronavirus. It interacts on the cell's surface with the serine protease TMPRSS2 and the surface receptor ACE2.
Native spike protein conists of a trimer that is based in the virus membrane. Therefore purifiy native & active SPIKE the trimer needs to be kept intact. Cube Biotech achieved this in three ways:
  1. MSP nanodiscs, as you can see here
  2. Detergent Mycelles
  3. Synthetic nanodiscs
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in MSP nanodisc, SDS Page Figure 1: SDS Page of purified SPIKE protein in MSP1D1 nanodiscs. The band that represents the MSP protein can also be seen.
 
SPIKE in MSP nanodisc, Gel filtration Figure 2: Gel filtration result of SPIKE in MSP nanodisc. The peak that represents spike is marked with a red arrow.

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl,
Nanodisc MSP1D1-His, assembly with POPC lipids
(Membrane scaffold protein based nanodisc; MSP1D1 = 25,3 kDa)
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity >Size exclusion chromatography, Superose 6 Increase, see Fig. 2
Related links to "SARS CoV-2 Spike protein nanodisc complex"
SPIKE in LMNG Mycelle, product image SARS CoV-2 spike protein in LMNG detergent
Article number: 28702
Sales price: From €754.00 *
COVID-19 antibody S2α (spike protein) 100 µg COVID-19 antibody S2α (spike protein) 100 µg
Article number: 28803
Sales price: €326.00 *
Product Image SPIKE protein in DIBMA SARS CoV-2 spike protein in DIBMA Glycerol
Article number: 28706
Sales price: From €986.00 *
SPIKE protein B.1.429 (CAl. 20C) SARS CoV-2 full-length Spike B.1.525 Mutation
Article number: 28733
Sales price: From €258.00 *
COVID-19 antibody RBD (spike protein) 100 µg COVID-19 antibody RBD (spike protein) 100 µg
Article number: 28802
Sales price: €326.00 *
SARS CoV-2 spike protein set in LMNG detergent (100 µg), DIBMA Glycerol (25 µg) and nanodisc complex SARS CoV-2 spike protein set in LMNG detergent...
Article number: 28704
Sales price: €2,654.00 *
SARS CoV-2 Spike B.1.351 Mutation RBD domain SARS CoV-2 Spike B.1.351 Mutation RBD domain
Article number: 28729
Sales price: From €552.00 *
COVID-19 Antibody S1 domain (spike protein) 100 µg COVID-19 Antibody S1 domain (spike protein) 100 µg
Article number: 28801
Sales price: €326.00 *
B.1.429 Corona mutant available at Cube Biotech SARS CoV-2 full-length Spike B.1.429 Mutation
Article number: 28736
Sales price: From €258.00 *
RB-Domain of the SARS-CoV 2 mutant strain P.1 SARS CoV-2 Spike P.1 Mutation RBD domain
Article number: 28731
Sales price: From €552.00 *
AChR T. Californica AChR T. Californica
Article number: 28601
Sales price: From €632.00 *
VGT protein VGT protein
Article number: 28501
Sales price: From €638.00 *
MSP1D1, lyophilized protein MSP1D1, lyophilized protein
Article number: 26132
Sales price: From €283.00 *
AASTY Screening Kit Contents AASTY Screening Kit (6x2x50 mg)
Article number: 18285
Sales price: €231.00 *
Vial of 25 µg of Omicron Spike protein SARS CoV-2 full-length Spike B.1.1.529 /...
Article number: 28750
Sales price: From €258.00 *
SARS CoV-2 full-length Spike B.1.621 (Mu) Mutation SARS CoV-2 full-length Spike B.1.621 (Mu) Mutation
Article number: 28747
Sales price: From €258.00 *
SARS-CoV-2 spike protein in SMALP 200 (25µg) SARS-CoV-2 spike protein in SMALP 200 (25µg)
Article number: 28715
Sales price: €986.00 *
HisCube Wash Buffer 2 X 50 mL HisCube Wash Buffer 2 X 50 mL
Article number: 80184
Sales price: €69.00 *
Nanodisc mouse MSP2N2-His_DMPG (50 µl) Nanodisc mouse MSP2N2-His_DMPG (50 µl)
Article number: 26775
Sales price: €627.00 *
Hs Sensory rhodopsin-1 Hs Sensory rhodopsin-1
Article number: 28911
Sales price: €356.00 *
NTSR1 protein NTSR1 protein
Article number: 28301
Sales price: From €1,085.00 *
GLP1R protein GLP1R protein
Article number: 28201
Sales price: From €1,205.00 *
alpaca MSP1D1-His, lyophilized protein alpaca MSP1D1-His, lyophilized protein
Article number: 27012
Sales price: From €167.00 *
Nanodisc Assembly Kit MSP2N2-His _POPC Nanodisc Assembly Kit MSP2N2-His _POPC
Article number: 26273
Sales price: €356.00 *
Cube MagBead Separator (1) Cube MagBead Separator (1)
Article number: 16941
Sales price: From €74.00 *
Spike Protein Delta Variant SARS CoV-2 full-length Spike B.1.617.2 (Delta)...
Article number: 28744
Sales price: From €258.00 *
RBD Domain of SPIKE from Mutant strain B.1.1.7 SARS CoV-2 Spike B.1.1.7 Mutation RBD domain
Article number: 28727
Sales price: From €552.00 *
SARS-CoV-2 spike protein in SMALP 140 (25 µg) SARS-CoV-2 spike protein in SMALP 140 (25 µg)
Article number: 28711
Sales price: €1,282.00 *
HisCube Binding Buffer 2 X 50 mL HisCube Binding Buffer 2 X 50 mL
Article number: 80181
Sales price: €69.00 *
HisCube - Ni-INDIGO His-Tag Protein Purification MINI Kit HisCube - Ni-INDIGO His-Tag Protein...
Article number: 80101
Sales price: €534.00 *
DIBMA Screening Kit, HEPES (4x3x50 mg) DIBMA Screening Kit, HEPES (4x3x50 mg)
Article number: 18101
Sales price: €231.00 *
Vial containing DIBMA 12 TRIS DIBMA 12, TRIS
Article number: 18014
Sales price: From €209.00 *
PureCube Compact Cartridge Fe-NTA PureCube Compact Cartridge Fe-NTA
Article number: 31602-Fe
Sales price: From €44.00 *
PureCube NHS Activated MagBeads XL PureCube NHS Activated MagBeads XL
Article number: 58405
Sales price: From €706.00 *
PureCube Rho1D4 Agarose XL PureCube Rho1D4 Agarose XL
Article number: 57601
Sales price: From €322.00 *
PureCube Co-NTA MagBeads XL PureCube Co-NTA MagBeads XL
Article number: 55505
Sales price: From €402.00 *
PureCube Ni-IDA Agarose XL PureCube Ni-IDA Agarose XL
Article number: 55003
Sales price: From €119.00 *
PureCube Co-NTA MagBeads PureCube Co-NTA MagBeads
Article number: 31501
Sales price: From €80.00 *
MC4R protein MC4R protein
Article number: 28401
Sales price: From €1,085.00 *
GPBAR1 (TGR5) protein GPBAR1 (TGR5) protein
Article number: 28101
Sales price: From €1,205.00 *
Viewed