SARS CoV-2 spike protein in LMNG detergent

€754.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

µG:

  • 28702
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE... more
Product information "SARS CoV-2 spike protein in LMNG detergent"
Product Type: Spike protein
Protein Sequence: Full-length
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways:
  1. MSP nanodiscs, based on MSP proteins
  2. Detergent Mycelles, as you can see here
  3. Synthetic nanodiscs
You can also purchase all three options together as a part of our practical 3 in 1 SPIKE protein set.

PDF Downloads

 
SPIKE in Degergent, SDS page, westernblot and Native GelFigure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.
A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in detergent mycelles..

Features

 
Alternative names SPIKE_SARS2 Spike glycoprotein
UniProt number P0DTC2
Protein class Single span transmembrane protein
Organism Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2)
Sequence
  • Full-length sequence (aa 1 – 1273),
  • furin cleavage site “RRAR” mutated to “GSAG”;
  • C-terminal Rho1D4 tag fused with spacer “GSSG” to protein sequence
Affinity tag C-terminal Rho1D4
Expression Host Hek293 Expi cells
Size 1286 amino acids (including Rho1D4 tag and linker)
142114 Da
Buffer composition 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG
Function host cell surface receptor binding; fusion of virus membrane with host endosome membrane
Purity (SDS-PAGE) >98% as determined by SDS-PAGE, see Fig. 1 A and B
Homogeneity Native Gel, see Fig. 1 C
Related links to "SARS CoV-2 spike protein in LMNG detergent"
Tube SARS CoV-2 full-length Spike B.1.1.7 Mutation
Article number: 28716
Sales price: From €258.00 *
tube containing SPIKE mutant protein SARS CoV-2 full-length Spike B.1.351 Mutation
Article number: 28719
Sales price: From €258.00 *
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
Article number: 40020
Sales price: €324.00 *
Vial of 25 µg of Omicron Spike protein SARS CoV-2 full-length Spike B.1.1.529 /...
Article number: 28750
Sales price: From €258.00 *
Overview of all Synthetic polymers that are contained in the MAXI screening Kit Synthetic Nanodisc Screening Kit MAXI (16x2x50 mg)
Article number: 18295
Sales price: €650.00 *
tube containing SPIKE mutant protein SARS CoV-2 full-length Spike B.1.351 Mutation
Article number: 28719
Sales price: From €258.00 *
PureCube Carboxy MagBeads XL PureCube Carboxy MagBeads XL
Article number: 58201
Sales price: From €128.00 *
AASTY 6-45 by Cube Biotech AASTY 6-45
Article number: 18250
Sales price: From €209.00 *
ACE2 Ectodomain by Cube Biotech ACE2 receptor (Ectodomain)
Article number: 28621
Sales price: From €291.00 *
OPRM1 protein nanodisc complex OPRM1 protein nanodisc complex
Article number: 29001
Sales price: From €1,838.00 *
n-Dodecyl-beta-maltoside (DDM) n-Dodecyl-beta-maltoside (DDM)
Article number: 16014
Sales price: From €293.00 *
Tube containing Ultrasolute™ Amphipol 18 Ultrasolute™ Amphipol 18 HEPES
Article number: 18321
Sales price: From €209.00 *
Tube Containing AASTY 11-55 AASTY 11-55
Article number: 18275
Sales price: From €209.00 *
AASTY Screening Kit Contents AASTY Screening Kit (6x2x50 mg)
Article number: 18285
Sales price: €231.00 *
SPIKE protein B.1.429 (CAl. 20C) SARS CoV-2 full-length Spike B.1.525 Mutation
Article number: 28733
Sales price: From €258.00 *
SARS-CoV-2 P.1 spike protein SARS CoV-2 full-length Spike P.1 Mutation
Article number: 28722
Sales price: From €258.00 *
Product Image SPIKE protein in DIBMA SARS CoV-2 spike protein in DIBMA Glycerol
Article number: 28706
Sales price: From €986.00 *
PureCube Butyl Agarose PureCube Butyl Agarose
Article number: 39003
Sales price: From €65.00 *
Ca Channelopsin 1 Ca Channelopsin 1
Article number: 28941
Sales price: €710.00 *
Nanodisc Assembly Kit MSP1E3D1-His_DMPC Nanodisc Assembly Kit MSP1E3D1-His_DMPC
Article number: 26251
Sales price: €285.00 *
Vial containing AASTY 6-50 AASTY 6-50
Article number: 18260
Sales price: From €209.00 *
B.1.429 Corona mutant available at Cube Biotech SARS CoV-2 full-length Spike B.1.429 Mutation
Article number: 28736
Sales price: From €258.00 *
RBD Domain of SPIKE from Mutant strain B.1.1.7 SARS CoV-2 Spike B.1.1.7 Mutation RBD domain
Article number: 28727
Sales price: From €552.00 *
Synthetic Nanodisc Screening Kit MINI, (8x2x50 mg) Synthetic Nanodisc Screening Kit MINI, (8x2x50 mg)
Article number: 18290
Sales price: €360.00 *
SMALP 200 SMALP 200
Article number: 18210
Sales price: From €209.00 *
VGT protein VGT protein
Article number: 28501
Sales price: From €638.00 *
PureCube Compact Cartridge Al-NTA PureCube Compact Cartridge Al-NTA
Article number: 31602-Al
Sales price: From €44.00 *
Detergent Screening Set Classic (1 g each of OG, LDAO, DM, DDM, OTG) Detergent Screening Set Classic (1 g each of...
Article number: 16092
Sales price: €240.00 *
Octylglucopyranoside (OG) Octylglucopyranoside (OG)
Article number: 16002
Sales price: From €164.00 *
AASTY 11-50 AASTY 11-50
Article number: 18265
Sales price: From €209.00 *
Tube containing AASTY 11-45 AASTY 11-45
Article number: 18255
Sales price: From €209.00 *
COVID-19 Antibody S1 domain (spike protein) 100 µg COVID-19 Antibody S1 domain (spike protein) 100 µg
Article number: 28801
Sales price: €326.00 *
SMALP Screening Set (4x3x50 mg) SMALP Screening Set (4x3x50 mg)
Article number: 18280
Sales price: €291.00 *
SARS CoV-2 spike protein set in LMNG detergent (100 µg), DIBMA Glycerol (25 µg) and nanodisc complex SARS CoV-2 spike protein set in LMNG detergent...
Article number: 28704
Sales price: €2,654.00 *
PureCube Zr-NTA MagBeads PureCube Zr-NTA MagBeads
Article number: 31501-Zr
Sales price: From €80.00 *
AChR T. Californica AChR T. Californica
Article number: 28601
Sales price: From €632.00 *
PureCube Compact Cartridge Fe-NTA PureCube Compact Cartridge Fe-NTA
Article number: 31602-Fe
Sales price: From €44.00 *
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Article number: 74103
Sales price: From €119.00 *
PureCube Amine Activated MagBeads XL PureCube Amine Activated MagBeads XL
Article number: 58905
Sales price: From €625.00 *
PureCube Epoxy Activated Agarose XL PureCube Epoxy Activated Agarose XL
Article number: 58703
Sales price: From €153.00 *
PureCube Rho1D4 Agarose XL PureCube Rho1D4 Agarose XL
Article number: 57601
Sales price: From €322.00 *
PureCube Maleimide Activated MagBeads PureCube Maleimide Activated MagBeads
Article number: 51201
Sales price: From €265.00 *
PureCube NHS Activated MagBeads PureCube NHS Activated MagBeads
Article number: 50405
Sales price: From €553.00 *
Viewed