SARS CoV-2 spike protein in LMNG detergent
- Order number: 28702
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE... more
Product information "SARS CoV-2 spike protein in LMNG detergent"
| Product Type: | Spike protein |
| Protein Sequence: | Full-length |
The coronavirus, also known as SARS-CoV-2, enters the cell by using its surface SPIKE. SPIKE is processed on the cell's surface by TMPRSS2, a serine protease. It then subsequently binds to ACE2 a cell surface receptor.
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways:
The Native SPIKE protein is a trimer that is located in the coronavirus membrane. Therefore to get pure & native SPIKE the trimer needs to be kept intact. Our lab staff achieved this in three different ways:
- MSP nanodiscs, based on MSP proteins
- Detergent Mycelles, as you can see here
- Synthetic nanodiscs
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Figure 1: Data from our lab regarding SPIKE stabilized in a detergent mycelle.A: SDS-Page of purified SPIKE in detergent mycelle.
B: Western blot of Rho1d4 tagged SPIKE, originated from the SDS Page seen in "A"
C: Native protein Gel of purified and stabilized SPIKE in detergent mycelles..
Features
| Alternative names | SPIKE_SARS2 Spike glycoprotein |
| UniProt number | P0DTC2 |
| Protein class | Single span transmembrane protein |
| Organism | Severe acute respiratory syndrome coronavirus 2 (2019-nCoV) (SARS-CoV-2) |
| Sequence |
|
| Affinity tag | C-terminal Rho1D4 |
| Expression Host | Hek293 Expi cells |
| Size | 1286 amino acids (including Rho1D4 tag and linker) 142114 Da |
| Buffer composition | 20 mM Hepes pH 7.5; 150 mM NaCl, 0.001 % LMNG |
| Function | host cell surface receptor binding; fusion of virus membrane with host endosome membrane |
| Purity (SDS-PAGE) | >98% as determined by SDS-PAGE, see Fig. 1 A and B |
| Homogeneity | Native Gel, see Fig. 1 C |
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