Rho1D4: The insider's tip for membrane protein purification

 

The problem

G protein-coupled receptors, like many other membrane proteins, are challenging to purify.

Expression levels are typically low, and more challenges arise from the use of detergents.

 

The solution

For over 20 years, researchers have successfully purified membrane proteins

with the Rho1D4 affinity tag.

 

 

Purification of a human GPCR from E.coli lysate on PureCube Rho1D4 Agarose
Purification of human GPCR from E.coli lysate on
PureCube Rho1D4 Agarose

 

 

 

Cube Biotech offers the first commercial, standardized Rho1D4 System.

 

 Get membrane proteins in high purity and yield
 Minimize variation in purification runs
 Avoid harsh elution conditions
 Conduct functional or conformational studies (how?)

 

 

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How do I get started?

Download our step-by-step protocols

Order a Starter Set,

with matrix and elution peptide at an exceptional price.

Starter Set 1 (bulk agarose)

Starter Set 2 (magbeads)

Starter Set 3 (prepacked cartridge)