CubeServices Protein Specific Matrices

In contrast to affinity chromatography or classical purification methods, protein-specific purification matrices enrich a protein via a specific interacting molecule. This molecule can be the natural ligand, an interacting protein or peptide, or a chemically synthesized drug. Through this specific interaction, proteins can be purified to homogeneity, not only by removal of impurities, but also by enriching for a particular protein conformation, e.g. an active form, or, as in the case of GPCRs, the agonist or antagonist conformation.
Protein-specific matrices are produced in our laboratory in Germany using a toolbox of materials and methods developed by our expert team. Our services include the following steps, of which individual packages can be chosen depending on requirements:
  • Expert design of coupling chemistry, including advice on matrix material, linkers, or spacers
  • Sourcing or production of ligands if applicable
  • Evaluation of the pilot batch
  • Production of the optimized protein-specific interaction matrix in sufficient quantities for your experiments
  • Electronic documentation, dedicated protocols and instructions to maximize output and purity
  • Reliable and optionally exclusive sourcing of the optimized purification matrix for future experiments
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