Guide to His affinity purification products

Guide to His affinity purification products

Scientists at Cube Biotech purify his-tagged proteins almost every day. New ideas how to optimize the procedure lead to novel products with new exciting features. However, it is difficult to keep track on new developments, and to decide which product to choose for a given application. Therefore, we have built two overview tables for Agaroses and MagBeads that -we hope- are helpful.
Please do not hesitate to contact us directly if you are still unsure, or if you are interested in customized or bulk products.
Table 1: Cube Biotech His Affinity Agarose products.

His Affinity Agarose

PureCube 100
INDIGO

Ni-Agarose

 

PureCube 100
Ni-NTA
Agarose

PureCube
Ni-NTA
Agarose
PureCube
Ni-NTA
Agarose

XL

PureCube
Ni-IDA
Agarose

Base material

PureCube 100 Agarose

PureCube 100 Agarose

PureCube Agarose

PureCube Agarose XL PureCube Agarose
Bead diameter 50-150 µm 50-150 µm

20-50 µm

300-500 µm 20-50 µm

Ligand

Proprietary NTA NTA NTA IDA

Binding capacity

up to 80 mg/ml up to 80 mg/ml up to 80 mg/ml >20 mg/ml 50 mg/ml

Purity

High High High High Medium

EDTA tolerance

20 mM 1 mM 1 mM 1 mM up to 1 mM

DTT tolerance

20 mM 10 mM 10 mM 10 mM up to 10 mM

pH tolerance

4-9 2-13 2-13 2-13 2-13

Cleaning in place

 

GuHCl, Urea, Ethanol

NaOH,

GuHCl, Urea, Ethanol

NaOH,

GuHCl, Urea, Ethanol

NaOH,

GuHCl, Urea, Ethanol

NaOH,

GuHCl, Urea, Ethanol

Metal stripping

No Yes Yes Yes Yes

Flow rate (batch/FPLC)

Fast Fast Medium n.a. Medium

Available metal ions

Ni Ni, Co Ni, Co, Al
Cu, Fe, Zn
Ni Ni

Special feature

NEW

High flow rates, EDTA-stable,

compatible

with eukaryotic growth media

NEW

High flow rates, High yields,

High purity

Standard material,

available with various

metal ions

For special applications

The economic alternative
Table 2: Cube Biotech His Affinity MagBead products.

His Affinity MagBeads

PureCube
INDIGO

Ni-MagBeads

PureCube
Ni-NTA
MagBeads

PureCube
Ni-NTA
MagBeads

XL
PureCube
Ni-IDA
MagBeads

 

Base material

PureCube

MagBeads

PureCube

MagBeads

PureCube

MagBeads XL

PureCube

MagBeads

Bead diameter 25 µm 25 µm 70-120 µm 25 µm

Ligand

Proprietary NTA NTA IDA

Binding capacity

up to 80 mg/ml up to 80 mg/ml >20 mg/ml >50 mg/ml

Purity

High High High Medium

EDTA tolerance

20 mM 1 mM 1 mM up to 1 mM

DTT tolerance

20 mM 10 mM 10 mM up to 10 mM

pH tolerance

4-9 2-13 2-13 2-13

 

Compatible cleaning agents

 

GuHCl, Urea,

Ethanol

NaOH,

GuHCl, Urea,

Ethanol

NaOH,

GuHCl, Urea,

Ethanol

NaOH,

GuHCl, Urea,

Ethanol

Metal stripping with EDTA

No Yes Yes Yes

Magnetic separation

Fast Fast Medium Fast

Available metal ions

Ni Ni, Co, Al
Cu, Fe, Zn
Ni Ni

Special feature

NEW

EDTA-stable, compatible

with eukaryotic growth media.

High yield & purity,

Broad ion variety

 

For special applications

Larger beads

on request

 

The economic alternative
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