IMAC increases sensitivity in Malaria diagnostics


Plasmodium cells in bloodstream

Immobilized metal affinity chromatography (IMAC) is a popular method for protein purification, particularly for his-tagged recombinant proteins.

But IMAC can be used in many other applications. Its high binding capacity towards the malaria biomarker protein HRP2 makes it an excellent enrichment method to increase sensitivity in malaria point-of-care diagnostic assays.

David W. Wright and his team at Vanderbilt University, Nashville, TN, USA have developed a novel device that efficiently combines proven point-of-care lateral flow assays with IMAC magnetic bead enrichment, which is deployed in collaboration with the Macha Research Trust in Zambia. In their work, Cube Biotech's Zn-NTA MagBeads showed highest biomarker protein recovery at lowest cost.


Download Application Note as pdf

Fig. 1: Cube Biotech's Zn-NTA MagBeads provides most efficient HRP2 capture at lowest cost.
Comparable volumes of IMAC MagBeads from four different manufacturers were evaluated as described in the application note.


               Download Application Note as pdf
  Zn-NTA MagBeads and other IMAC products
  Learn more about IMAC
  Learn more about MagBeads

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