We produce and use our own PureCube MagBeads. With a ferrimagnetic core and an agarose coating coupled to a ligand of choice, their surface is identical to our PureCube Agarose
matrices, making them an ideal combination for all purification scales. In addition, the amount of magnetic beads used for a purification setup can be easily scaled up and down to match protein expression rates and culture volumes. Available volumes range from 1 ml to bulk scale at affordable pricing.
PureCube MagBeads are useful to:
extract proteins from diluted solutions, such as cell culture supernatants
purify proteins expressed at low levels
perform pull-down experiments
automate small-scale protein purification
The GST tag
is a 26 kDa protein tag which is often chosen to promote recombinant protein folding.
is a 8 amino acid tag with high specificity for StrepTactin® magbeads which is often used as alternative or in combination with the His tag
is a 9 amino acid tag that binds to the rho1D4 antibody and has proven particularly efficient in the purification of membrane proteins. For the first time commercially available, Cube Biotech offers the full system (affinity magbeads, rho1D4 peptide, and rho1D4 antibody).
can be used to couple your own antibody or other molecule to create a protein-specific affinity matrix. Available modifications include:
can be used for a combination of magnetic and filter separation during purification, and for other special applications.
Special and Custom Matrices can be used for tag-free purification, purification of native proteins and specific protein conformations. Learn more.
StrepTactin® and Strep-tag® are trademarks of IBA GmbH.