PureCube Glutathione MagBeads | Cube Biotech

GST Affinity MagBeads


MAGBeads;magnetic_beads
Magnetic beads are useful to purify proteins from dilute solutions, or when only low expression levels are achieved. PureCube Glutathione MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins. PureCube MagBeads are produced in a very robust, reproducible way to minimize lot-to-lot variability. (Fig.1) Our MagBeads are homogeneous in size, and provide the identical surface chemistry as PureCube Glutathione Agarose, making magnetic beads an excellent tool for fast screening of binding conditions. PureCube Glutathione MagBeads are provided as a 25% suspension.

PureCube Glutathione MagBeads provide:

  • Binding capacity of up to 12 mg protein per ml settled beads
  • purification of proteins expressed at low levels or from diluted solutions
  • optimal conditions for pull-down experiments
  • low unspecific binding
  • Also available as Glutathione Agarose and prepacked cartridges

PureCube Gluathione MagBead's efficiency

GST
Fig.1.: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.
Table 1: Overview of commercially available Glutathione MagBeads.
 Cube BiotechSupplier PSupplier S
Material Magnetite (Fe3O4) beads coated with 6% cross- linked agarose Iron oxide particles encapsulated in crosslinked agarose 4% beaded agarose
Affinity ligand Glutathione covalently coupled to Agarose via a neutral 12 atom spacer Reduced glutathione (GSH) covalently attached via sulfide linkage using a 12-atom linker Glutathione covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom spacer
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 1 to 10 µm 20-75 µm
Binding capacity 12 mg GST/mL of settled beads 5 to 10 mg GST/mL of settled beads ≥15 μmol/mL agarose
Concentration 25% slurry in 20% ethanol 25% slurry in 20% ethanol 50% slurry in 30% ethanol
 
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GST Affinity MagBeads
MAGBeads;magnetic_beads
Magnetic beads are useful to purify proteins from dilute solutions, or when only low expression levels are achieved. PureCube Glutathione MagBeads are ferrimagnetic agarose beads coupled to glutathione, an efficient ligand for GST fusion proteins. PureCube MagBeads are produced in a very robust, reproducible way to minimize lot-to-lot variability. (Fig.1) Our MagBeads are homogeneous in size, and provide the identical surface chemistry as PureCube Glutathione Agarose, making magnetic beads an excellent tool for fast screening of binding conditions. PureCube Glutathione MagBeads are provided as a 25% suspension.

PureCube Glutathione MagBeads provide:

  • Binding capacity of up to 12 mg protein per ml settled beads
  • purification of proteins expressed at low levels or from diluted solutions
  • optimal conditions for pull-down experiments
  • low unspecific binding
  • Also available as Glutathione Agarose and prepacked cartridges

PureCube Gluathione MagBead's efficiency

GST
Fig.1.: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.
Table 1: Overview of commercially available Glutathione MagBeads.
 Cube BiotechSupplier PSupplier S
Material Magnetite (Fe3O4) beads coated with 6% cross- linked agarose Iron oxide particles encapsulated in crosslinked agarose 4% beaded agarose
Affinity ligand Glutathione covalently coupled to Agarose via a neutral 12 atom spacer Reduced glutathione (GSH) covalently attached via sulfide linkage using a 12-atom linker Glutathione covalently attached through the sulfur to epoxy-activated agarose resulting in a 12 atom spacer
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 1 to 10 µm 20-75 µm
Binding capacity 12 mg GST/mL of settled beads 5 to 10 mg GST/mL of settled beads ≥15 μmol/mL agarose
Concentration 25% slurry in 20% ethanol 25% slurry in 20% ethanol 50% slurry in 30% ethanol
 
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GST Affinity MagBeads
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PureCube Glutathione MagBeads PureCube Glutathione MagBeads
1 ml 25% Glutathione Magnetic Beads For more information read here: https://cube-biotech.com/products/magnetic-beads/gst-affinity-magbeads/#
Article number: 32201
Sales price: From €49.00 *
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