PureCube His Affinity MagBeads | Cube Biotech

His Affinity MagBeads


MagBeads
Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. PureCube MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel, cobalt, or other ions. In addition, the novel INDIGO ligand provides optimal performance even in the presence of 20 mM EDTA and DTT. Both ligand-metal systems efficiently bind histidine-tagged proteins. The agarose surface is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions.All PureCube His Affinity MagBeads are delivered as a 25% suspension.

PureCube His Affinity MagBeads provide:

  • Binding capacity of up to 80 mg/mL (for Ni-NTA)
  • Low unspecific binding
  • Purification of proteins expressed at low levels or from diluted solutions
  • Highly reproducible, careful in-house production

Table 1: Features of commercially available His affinity magnetic beads.

 Cube BiotechSupplier GSupplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross-linked agarose Highly cross-linked spherical agarose including magnetite Agarose beads with magnetic particles
Affinity ligand INDIGO, Ni-NTA, Co-NTA, NTA Cu-NTA, Al-NTA, Zn-NTA Ni-IDA, IDA Ni-IMAC NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 80 mg His-tagged protein/mL of settled beads 50 mg His-tagged protein/mL of settled beads 2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol

NTA IDA Protein binding; purification
IDA vs NTA
As depicted in Figure 1, the chelating ligands iminodiacetic acid (IDA) and nitrilotriacetic acid (NTA) support similar interaction between nickel and the imidazole rings of the histidine tag. The ligands differ in size on the one hand; the smaller IDA couples to the resin at a higher density and therefore has higher binding capacity. On othe other hand, IDA coordinates the nickel ion with one less valency than NTA (orange circles). As a result, more metal ions may leach from IDA resins giving a less pure eluted protein. Learn more
Fig.1: NTA vs. IDA. Chelating ligands nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) support similar interaction between Ni2+ and imidiazole rings of a polyhistidine tag, but NTA coordinates the Ni2+ with 4 valencies and IDA with only 3 (orange circles). This difference impacts the quality of the resulting purified protein fraction.
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His Affinity MagBeads
MagBeads
Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. PureCube MagBeads are ferrimagnetic agarose beads coupled to a chelating ligand (IDA or NTA) coordinating nickel, cobalt, or other ions. In addition, the novel INDIGO ligand provides optimal performance even in the presence of 20 mM EDTA and DTT. Both ligand-metal systems efficiently bind histidine-tagged proteins. The agarose surface is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions.All PureCube His Affinity MagBeads are delivered as a 25% suspension.

PureCube His Affinity MagBeads provide:

  • Binding capacity of up to 80 mg/mL (for Ni-NTA)
  • Low unspecific binding
  • Purification of proteins expressed at low levels or from diluted solutions
  • Highly reproducible, careful in-house production

Table 1: Features of commercially available His affinity magnetic beads.

 Cube BiotechSupplier GSupplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross-linked agarose Highly cross-linked spherical agarose including magnetite Agarose beads with magnetic particles
Affinity ligand INDIGO, Ni-NTA, Co-NTA, NTA Cu-NTA, Al-NTA, Zn-NTA Ni-IDA, IDA Ni-IMAC NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 80 mg His-tagged protein/mL of settled beads 50 mg His-tagged protein/mL of settled beads 2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol

NTA IDA Protein binding; purification
IDA vs NTA
As depicted in Figure 1, the chelating ligands iminodiacetic acid (IDA) and nitrilotriacetic acid (NTA) support similar interaction between nickel and the imidazole rings of the histidine tag. The ligands differ in size on the one hand; the smaller IDA couples to the resin at a higher density and therefore has higher binding capacity. On othe other hand, IDA coordinates the nickel ion with one less valency than NTA (orange circles). As a result, more metal ions may leach from IDA resins giving a less pure eluted protein. Learn more
Fig.1: NTA vs. IDA. Chelating ligands nitrilotriacetic acid (NTA) and iminodiacetic acid (IDA) support similar interaction between Ni2+ and imidiazole rings of a polyhistidine tag, but NTA coordinates the Ni2+ with 4 valencies and IDA with only 3 (orange circles). This difference impacts the quality of the resulting purified protein fraction.
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PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
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PureCube Co-NTA MagBeads PureCube Co-NTA MagBeads
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PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
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PureCube IDA MagBeads PureCube IDA MagBeads
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PureCube NTA MagBeads PureCube NTA MagBeads
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His Affinity MagBeads
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PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
1 ml 25% magnetic beads equipped with a proprietary ligand for His affinity purification, loaded with nickel (II) sulfate For more information read here: https://cube-biotech.com/products/magnetic-beads/his-affinity-magbeads/#
Article number: 75201
Sales price: From €67.00 *
PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
1 ml 25% NTA magnetic beads, loaded with nickel (II) sulfate
Article number: 31201
Sales price: From €61.00 *
PureCube Co-NTA MagBeads PureCube Co-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with cobalt (II) chloride
Article number: 31501
Sales price: From €66.00 *
PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
1 ml 25% IDA magnetic beads, loaded with nickel (II) sulfate
Article number: 30201
Sales price: From €42.00 *
PureCube IDA MagBeads PureCube IDA MagBeads
1 ml 25% IDA Magnetic Beads - for loading with transition metals
Article number: 30801
Sales price: From €42.00 *
PureCube NTA MagBeads PureCube NTA MagBeads
1 ml 25% NTA Magnetic Beads - for loading with transition metals
Article number: 31801
Sales price: From €61.00 *
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