PureCube Ni-IDA MagBeads | Cube Biotech

Ni-IDA MagBeads


MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-IDA MagBeads are ferrimagnetic agarose beads coupled to an IDA chelating ligand, loaded with nickel ions. PureCube Ni-NTA MagBeads are delivered as a 25% suspension. For increased specificity, we offer a range of different His affinity resins, e.g. carrying the NTA ligand (what's the difference?). Please see our overview page for more information.

PureCube Ni-IDA MagBeads offer:

  • High binding capacity (up to 70 mg/mL settled beads)
  • Low unspecific binding
  • Stable in up to 10 mM DTT and up to 1 mM EDTA (Not stable enough?)
  • Ideal for purification from dilute solutions and for pulldown experiments
  • Also available as Ni-IDA Agarose and prepacked cartridges

Table 1: Features of commercially available His affinity magnetic beads.

 Cube BiotechSupplier GSupplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross- linked agarose Highly cross-linked spherical agarose including magnetite Agarose beads with magnetic particles
Affinity ligand Ni-IDA, NTA loaded with Ni, Co, Al, Cu or Zn NEW: INDIGO Ni-MagBeads Ni-IMAC Ni-NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads 50 mg His-tagged protein/mL of settled beads 2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol
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Ni-IDA MagBeads
MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-IDA MagBeads are ferrimagnetic agarose beads coupled to an IDA chelating ligand, loaded with nickel ions. PureCube Ni-NTA MagBeads are delivered as a 25% suspension. For increased specificity, we offer a range of different His affinity resins, e.g. carrying the NTA ligand (what's the difference?). Please see our overview page for more information.

PureCube Ni-IDA MagBeads offer:

  • High binding capacity (up to 70 mg/mL settled beads)
  • Low unspecific binding
  • Stable in up to 10 mM DTT and up to 1 mM EDTA (Not stable enough?)
  • Ideal for purification from dilute solutions and for pulldown experiments
  • Also available as Ni-IDA Agarose and prepacked cartridges

Table 1: Features of commercially available His affinity magnetic beads.

 Cube BiotechSupplier GSupplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross- linked agarose Highly cross-linked spherical agarose including magnetite Agarose beads with magnetic particles
Affinity ligand Ni-IDA, NTA loaded with Ni, Co, Al, Cu or Zn NEW: INDIGO Ni-MagBeads Ni-IMAC Ni-NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads 50 mg His-tagged protein/mL of settled beads 2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol
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PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
Article number: 30201
Ni-IDA MagBeads
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PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
1 ml 25% IDA magnetic beads, loaded with nickel (II) sulfate
Article number: 30201
Sales price: From €42.00 *
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