PureCube Ni-NTA MagBeads | Cube Biotech

Ni-NTA MagBeads


MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.

Why PureCube Ni-NTA MagBeads?

Table 1: Features of commercially available His affinity magnetic beads.

 Cube BiotechSupplier GSupplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross-linked agarose Highly cross-linked spherical agarose including magnetite Agarose beads with magnetic particles
Affinity ligand Ni-IDA, NTA loaded with Ni, Co, Al, Cu or Zn NEW: INDIGO Ni-MagBeads Ni-IMAC Ni-NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads 50 mg His-tagged protein/mL of settled beads 2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol
Ni-NTA_vs_Binding_Capacity
 
Fig.1: 46% higher yield obtained with PureCube Ni-NTA MagBeads. SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of PureCube Ni-NTA MagBeads and equivalent, market-leading magnetic beads.
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Ni-NTA MagBeads
MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.

Why PureCube Ni-NTA MagBeads?

Table 1: Features of commercially available His affinity magnetic beads.

 Cube BiotechSupplier GSupplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross-linked agarose Highly cross-linked spherical agarose including magnetite Agarose beads with magnetic particles
Affinity ligand Ni-IDA, NTA loaded with Ni, Co, Al, Cu or Zn NEW: INDIGO Ni-MagBeads Ni-IMAC Ni-NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads 50 mg His-tagged protein/mL of settled beads 2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol
Ni-NTA_vs_Binding_Capacity
 
Fig.1: 46% higher yield obtained with PureCube Ni-NTA MagBeads. SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of PureCube Ni-NTA MagBeads and equivalent, market-leading magnetic beads.
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PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
Article number: 31201
Ni-NTA MagBeads
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PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
1 ml 25% NTA magnetic beads, loaded with nickel (II) sulfate
Article number: 31201
Sales price: From €61.00 *
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