The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose
, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.
Why PureCube Ni-NTA MagBeads?
Table 1: Features of commercially available His affinity magnetic beads.
| ||Cube Biotech||Supplier G||Supplier Q|
||Magnetite (Fe3O4) beads coated with 6% cross-linked agarose
||Highly cross-linked spherical agarose including magnetite
||Agarose beads with magnetic particles
||Ni-IDA, NTA loaded with Ni, Co, Al, Cu or Zn NEW: INDIGO Ni-MagBeads
||63 or 37-100 µm
||up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads
||50 mg His-tagged protein/mL of settled beads
||2 mg His-tagged protein/ml suspension (40 mg/ml settled beads)
||25% slurry in 20% ethanol
||10 or 5% slurry in 20% ethanol
||5% slurry in 30% ethanol
Fig.1: 46% higher yield obtained with PureCube Ni-NTA MagBeads. SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of PureCube Ni-NTA MagBeads and equivalent, market-leading magnetic beads.