PureCube Ni-NTA MagBeads


PureCube Ni-NTA MagBeads

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.
MAGEBEADS
HIS
 
Why PureCube Ni-NTA MagBeads?
Highest binding capacity on the market (up to 80 mg/mL settled beads)
Low unspecific binding
Ideal for purification from dilute solutions and for pulldown experiments
Also available as Ni-NTA Agarose and prepacked cartridges
Learn_IMAC_CubeColors
Learn_MagBeads_CubeColors
  Cube Biotech Supplier G Supplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross-linked agarose Highly cross-linked spherical agarose including magnetite

Agarose beads with

magnetic particles

Affinity ligand

Ni-IDA,

NTA loaded with

Ni, Co, Al, Cu or Zn

NEW: INDIGO Ni-MagBeads

Ni-IMAC Ni-NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads

50 mg His-tagged

protein/mL of settled beads

2 mg His-tagged

protein/ml suspension

(40 mg/ml settled beads)

Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol
Table 1: Features of commercially available His affinity magnetic beads.
 
Fig.1: >46% higher yield obtained with PureCube Ni-NTA MagBeads. SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of PureCube Ni-NTA MagBeads and equivalent, market-leading magnetic beads.
Close window
PureCube Ni-NTA MagBeads

PureCube Ni-NTA MagBeads

The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube Ni-NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand, loaded with nickel ions. They are delivered as a 25% suspension.
MAGEBEADS
HIS
 
Why PureCube Ni-NTA MagBeads?
Highest binding capacity on the market (up to 80 mg/mL settled beads)
Low unspecific binding
Ideal for purification from dilute solutions and for pulldown experiments
Also available as Ni-NTA Agarose and prepacked cartridges
Learn_IMAC_CubeColors
Learn_MagBeads_CubeColors
  Cube Biotech Supplier G Supplier Q
Material Magnetite (Fe3O4) beads coated with 6% cross-linked agarose Highly cross-linked spherical agarose including magnetite

Agarose beads with

magnetic particles

Affinity ligand

Ni-IDA,

NTA loaded with

Ni, Co, Al, Cu or Zn

NEW: INDIGO Ni-MagBeads

Ni-IMAC Ni-NTA
Magnetization ferrimagnetic ferrimagnetic ferrimagnetic
Bead diameter 25-30 µm 63 or 37-100 µm 20-70 µm
Binding capacity up to 70 mg (IDA) or 80 mg (NTA/INDIGO) His-tagged protein/mL of settled beads

50 mg His-tagged

protein/mL of settled beads

2 mg His-tagged

protein/ml suspension

(40 mg/ml settled beads)

Concentration 25% slurry in 20% ethanol 10 or 5% slurry in 20% ethanol 5% slurry in 30% ethanol
Table 1: Features of commercially available His affinity magnetic beads.
 
Fig.1: >46% higher yield obtained with PureCube Ni-NTA MagBeads. SDS-PAGE of GFP expressed in E.coli and purified with comparable amounts of PureCube Ni-NTA MagBeads and equivalent, market-leading magnetic beads.
Top seller
PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
Article number: 31201
Ni-NTA MagBeads
Close filters
 
  •  
No results were found for the filter!
PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
1 ml 25% NTA magnetic beads, loaded with nickel (II) sulfate
Article number: 31201
Sales price: From €61.00 *
Viewed