NTA MagBeads


MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand. NTA MagBeads can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. PureCube NTA MagBeads are delivered as a 25% suspension. Do you prefer IDA? We offer the our MagBeads also with that chelating ligand! (what's the difference?)

Why PureCube NTA MagBeads?


Features

Usage Unloaded NTA MagBeads 7 magnetic Beads for costumized purpose
Specifity Depending of loaded metal ion (E.g. Nickel for His-affinity)
Protein Binding capacity 40 mg / ml
Stability Stable in pH2-14; 100% methanol, 100% Ethanol, 8M urea, 6M Guanidinium hydrochloride, 30% acetonitrile
Particle diameter 30 µm
Filling quantity Delivered as a 25 % suspension
Required equipment
 
  • Sodium acetate trihydrate
  • Ethanol
  • Hydrochloric acid
  • Al(III)chloride, Co(II)chloride, Cu(II)chloride,Fe(III)chloride, or Zn(II)chloride
  • Nickel II sulfate
  • Sodium chloride
  • Acetic acid
  • Wash Buffer
  • Centrifuge 50 mL Tubes
  • Vortex mixer
  • End-over-end shaker
  • Magnetic separator for microcentrifuge tubes
  • Vortex mixer

Applications - loading of the Agarose with metal ions

All and buffer compositions protocols are also avaible as PDF-Files on the Protocols & Datasheets page.
   
A.Protocol to load Nickel
 
 
  1. Transfer 1 mL PureCube NTA MagBeads into a 2 mL microcentrifuge tube.
  2. Tip: The loading reaction can be scaled up and down linearly, by increasing or decreasing the amounts of buffers and solutions described in this protocol.
  3. Place the tube on a magnetic stand and allow the beads to separate. Remove the supernatant. Resuspend the magnetic beads with 1 mL double distilled water.
  4. Separate the beads and wash two more times with water.
  5. Wash 3x with 50 mM sodium acetate, pH 6.0.
  6. Wash 1x with double distilled water.
  7. Add 1 ml 2.5% nickel sulfate solution and incubate for 2 h on an end-over-end shaker.
  8. Wash 4x with double distilled water.
  9. Add 1 mL Wash Buffer and incubate for 10 min.
  10. Wash 1x with double distilled water.
  11. Wash 6x with 20 mM Tris-HCl, pH 7.5.
  12. Wash 1x with double distilled water.
  13. . Resuspend the now metal-ion loaded Ni-NTA MagBeads in 1 mL MagBead Storage buffer, yielding a 25% suspension. Store at 4°C.
 B.Protocol to load Co, Cu, AL, Fe; Zn and other metals
 
 
  1. Transfer 1 mL PureCube NTA or IDA MagBeads into a 2 mL microcentrifuge tube. Tip: The loading reaction can be scaled up and down linearly, by increasing or decreasing the amounts of buffers and solutions described in this protocol.
  2. Place the tube on a magnetic stand and allow the beads to separate. Remove the supernatant. Resuspend the magnetic beads with 1 mL double distilled water.
  3. Separate the beads and wash two more times with water.
  4. Wash 3x with 50 mM sodium acetate, pH 6.0.
  5. Wash 1x with 20 mL double distilled water.
  6. Add 20 ml 2.5% transition metal solution and incubate for 2 h on an end-over-end shaker.
  7. Wash 4x with 20 mL double distilled water.
  8. Wash 6x with 20 mL 20 mM Tris-HCl, pH 7.5.
  9. Wash 1x with 20 mL double distilled water.
  10. Resuspend the now metal-ion loaded NTA magbeads in 1 mL MagBead Storage buffer, yielding a 25% suspension. Store at 4°C.
MagBeads
The His tag is the most widely used affinity tag due to its small size and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. The agarose surface of our MagBeads is identical to that of PureCube Agarose, making them an ideal combination for small-scale screening and upscale reactions. PureCube NTA MagBeads are ferrimagnetic agarose beads coupled to an NTA chelating ligand. NTA MagBeads can be loaded with transition metals to obtain different specificities for his-tagged proteins, or for other applications such as purification of phosphorylated proteins. PureCube NTA MagBeads are delivered as a 25% suspension. Do you prefer IDA? We offer the our MagBeads also with that chelating ligand! (what's the difference?)

Why PureCube NTA MagBeads?


Features

Usage Unloaded NTA MagBeads 7 magnetic Beads for costumized purpose
Specifity Depending of loaded metal ion (E.g. Nickel for His-affinity)
Protein Binding capacity 40 mg / ml
Stability Stable in pH2-14; 100% methanol, 100% Ethanol, 8M urea, 6M Guanidinium hydrochloride, 30% acetonitrile
Particle diameter 30 µm
Filling quantity Delivered as a 25 % suspension
Required equipment
 
  • Sodium acetate trihydrate
  • Ethanol
  • Hydrochloric acid
  • Al(III)chloride, Co(II)chloride, Cu(II)chloride,Fe(III)chloride, or Zn(II)chloride
  • Nickel II sulfate
  • Sodium chloride
  • Acetic acid
  • Wash Buffer
  • Centrifuge 50 mL Tubes
  • Vortex mixer
  • End-over-end shaker
  • Magnetic separator for microcentrifuge tubes
  • Vortex mixer

Applications - loading of the Agarose with metal ions

All and buffer compositions protocols are also avaible as PDF-Files on the Protocols & Datasheets page.
   
A.Protocol to load Nickel
 
 
  1. Transfer 1 mL PureCube NTA MagBeads into a 2 mL microcentrifuge tube.
  2. Tip: The loading reaction can be scaled up and down linearly, by increasing or decreasing the amounts of buffers and solutions described in this protocol.
  3. Place the tube on a magnetic stand and allow the beads to separate. Remove the supernatant. Resuspend the magnetic beads with 1 mL double distilled water.
  4. Separate the beads and wash two more times with water.
  5. Wash 3x with 50 mM sodium acetate, pH 6.0.
  6. Wash 1x with double distilled water.
  7. Add 1 ml 2.5% nickel sulfate solution and incubate for 2 h on an end-over-end shaker.
  8. Wash 4x with double distilled water.
  9. Add 1 mL Wash Buffer and incubate for 10 min.
  10. Wash 1x with double distilled water.
  11. Wash 6x with 20 mM Tris-HCl, pH 7.5.
  12. Wash 1x with double distilled water.
  13. . Resuspend the now metal-ion loaded Ni-NTA MagBeads in 1 mL MagBead Storage buffer, yielding a 25% suspension. Store at 4°C.
 B.Protocol to load Co, Cu, AL, Fe; Zn and other metals
 
 
  1. Transfer 1 mL PureCube NTA or IDA MagBeads into a 2 mL microcentrifuge tube. Tip: The loading reaction can be scaled up and down linearly, by increasing or decreasing the amounts of buffers and solutions described in this protocol.
  2. Place the tube on a magnetic stand and allow the beads to separate. Remove the supernatant. Resuspend the magnetic beads with 1 mL double distilled water.
  3. Separate the beads and wash two more times with water.
  4. Wash 3x with 50 mM sodium acetate, pH 6.0.
  5. Wash 1x with 20 mL double distilled water.
  6. Add 20 ml 2.5% transition metal solution and incubate for 2 h on an end-over-end shaker.
  7. Wash 4x with 20 mL double distilled water.
  8. Wash 6x with 20 mL 20 mM Tris-HCl, pH 7.5.
  9. Wash 1x with 20 mL double distilled water.
  10. Resuspend the now metal-ion loaded NTA magbeads in 1 mL MagBead Storage buffer, yielding a 25% suspension. Store at 4°C.
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PureCube NTA MagBeads PureCube NTA MagBeads
Article number: 31801
NTA MagBeads
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PureCube NTA MagBeads PureCube NTA MagBeads
1 ml 25% NTA Magnetic Beads - for loading with transition metals
Article number: 31801
Sales price: From €61.00 *
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