Al-NTA MagBeads


MagBeads
Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. Al-NTA MagBeads are often used for the purification of phosphorylated proteins. Alternative matrices are Fe-, Ga- or Zr-NTA. All PureCube IMAC MagBeads are delivered as a 25% suspension.

PureCube Al-NTA MagBeads provide:

  • Ready-to-use magnetic beads
  • Purification of phosphorylated proteins
  • Al-NTA Agaroses also available

Purification of phosphorylated proteins

IMAC methods, in particular Al-IDA, Fe-NTA or Ga-NTA, have been widely used to enrich phosphoproteins and phosphopeptides as part of the sample preparation for mass spectrometry (1,3,4). Depending on the sample, and the kind of phosphorylated proteins to be analyzed, also other transition metals, such as zirconium or aluminium have been loaded on NTA or IDA matrices for enrichment (5). Also, magnetic beads have been useful for this kind of sample preparation (2).

Features

Usage Specific binding of phosphorylated proteins
Specifity Affinity to phosphorylated proteins
Binding capacity Depending on the application
Filling quantity Delivered as a 25 % suspension
Bead size 30 μm
Metal Ion capacity >12 µeqv Al3+/mL.
Bead Ligand Al-NTA

Literature references
1. Albuquerque, C.P. et al. A multidimensional chromatography technology for in-depth phosphoproteome analysis. Mol Cell Proteomics (2008), 7(7), 1389-1396.
2. Herskowitz, J., et al. Phosphoproteomic analysis reveals site-specific changes in GFAP and NDGR2 phosphorylation in frontotemporal lobar degeneration. J. Proteome Res (2010), 9(12):6368-6379.
3. Yu, P. et al. Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans. PLoS One (2013), 8,3, e59285.
4. Aryal, U.K. et al. Optimization of immobilized Gallium (III) ion affinity chromatography for selective binding and recovery of phosphopeptides from protein digests. Journal of Biomolecular Techniques (2008), 19:296-310.
5. Block et. al. Immobilized-metal affinity chromatography (IMAC) a review. Methods Enzymol. (2009), 463:439-73.
MagBeads
Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. Al-NTA MagBeads are often used for the purification of phosphorylated proteins. Alternative matrices are Fe-, Ga- or Zr-NTA. All PureCube IMAC MagBeads are delivered as a 25% suspension.

PureCube Al-NTA MagBeads provide:

  • Ready-to-use magnetic beads
  • Purification of phosphorylated proteins
  • Al-NTA Agaroses also available

Purification of phosphorylated proteins

IMAC methods, in particular Al-IDA, Fe-NTA or Ga-NTA, have been widely used to enrich phosphoproteins and phosphopeptides as part of the sample preparation for mass spectrometry (1,3,4). Depending on the sample, and the kind of phosphorylated proteins to be analyzed, also other transition metals, such as zirconium or aluminium have been loaded on NTA or IDA matrices for enrichment (5). Also, magnetic beads have been useful for this kind of sample preparation (2).

Features

Usage Specific binding of phosphorylated proteins
Specifity Affinity to phosphorylated proteins
Binding capacity Depending on the application
Filling quantity Delivered as a 25 % suspension
Bead size 30 μm
Metal Ion capacity >12 µeqv Al3+/mL.
Bead Ligand Al-NTA

Literature references
1. Albuquerque, C.P. et al. A multidimensional chromatography technology for in-depth phosphoproteome analysis. Mol Cell Proteomics (2008), 7(7), 1389-1396.
2. Herskowitz, J., et al. Phosphoproteomic analysis reveals site-specific changes in GFAP and NDGR2 phosphorylation in frontotemporal lobar degeneration. J. Proteome Res (2010), 9(12):6368-6379.
3. Yu, P. et al. Global analysis of neuronal phosphoproteome regulation by chondroitin sulfate proteoglycans. PLoS One (2013), 8,3, e59285.
4. Aryal, U.K. et al. Optimization of immobilized Gallium (III) ion affinity chromatography for selective binding and recovery of phosphopeptides from protein digests. Journal of Biomolecular Techniques (2008), 19:296-310.
5. Block et. al. Immobilized-metal affinity chromatography (IMAC) a review. Methods Enzymol. (2009), 463:439-73.
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PureCube Al-NTA MagBeads PureCube Al-NTA MagBeads
Article number: 31501-Al
Al-NTA MagBeads
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PureCube Al-NTA MagBeads PureCube Al-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with aluminium (III) chloride
Article number: 31501-Al
Sales price: From €66.00 *
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