Zn-NTA MagBeads


MagBeads
Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA and IDA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. Zn-NTA MagBeads are the optimal matrix for the purification of zinc-finger proteins, either via his tag, or via direct interaction of the zinc-binding motif with the NTA ligand. All PureCube IMAC MagBeads are delivered as a 25% suspension.

PureCube Zn-NTA MagBeads provide:

  • Ready-to-use magnetic beads
  • Purification of zinc-finger proteins, with or without his tag
  • Purification of his-tagged proteins with different specificity
  • Zn-NTA Agaroses also available
Zn-NTA MagBeads and Agaroses have been successfully used to enhance sensitivity in Malaria detection. Read our application note to learn more.

Purification of zinc-finger proteins

When purifying his-tagged zinc-finger proteins which require a bound zinc ion for activity, it is often advisable to use Zn-IMAC instead of Ni-IMAC materials. Zinc matrices provide a high specificity for his-tagged protein purification (see Fig. 1) and an exchange of zinc bound to the active site of the protein by nickel is avoided (2). At the same time, non-tagged zinc-finger proteins can be purified using zinc IMAC matrices, simply by their affinity to this metal (1).

Features

Usage Specific binding of his tagged and zinc finger proteins
Specifity Affinity to his tagged and zinc finger proteins
Binding capacity Depending on the application
Filling quantity Delivered as a 25 % suspension
Bead size 30 μm
Metal Ion capacity >12 µeqv Zn2+/mL.
Bead Ligand Zn-NTA

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
metal ions affinity to his tag
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Literature references
1. Vorácková, I. et al. Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography. Protein Expr. Purif. (2011) 79(1):88-95.
2. Block et. al. Immobilized-metal affinity chromatography (IMAC) a review. Methods Enzymol. (2009), 463:439-73.
MagBeads
Immobilized metal affinity chromatography, or IMAC, has been employed for many applications making use of its versatile interaction with aromatic nitrogens and phosphates. To this end, NTA and IDA matrices have been loaded with a broad range of transition metals, as optimized for the individual interaction. Magnetic beads are ideal for protein purification from dilute supernatants and for pull-down experiments. Zn-NTA MagBeads are the optimal matrix for the purification of zinc-finger proteins, either via his tag, or via direct interaction of the zinc-binding motif with the NTA ligand. All PureCube IMAC MagBeads are delivered as a 25% suspension.

PureCube Zn-NTA MagBeads provide:

  • Ready-to-use magnetic beads
  • Purification of zinc-finger proteins, with or without his tag
  • Purification of his-tagged proteins with different specificity
  • Zn-NTA Agaroses also available
Zn-NTA MagBeads and Agaroses have been successfully used to enhance sensitivity in Malaria detection. Read our application note to learn more.

Purification of zinc-finger proteins

When purifying his-tagged zinc-finger proteins which require a bound zinc ion for activity, it is often advisable to use Zn-IMAC instead of Ni-IMAC materials. Zinc matrices provide a high specificity for his-tagged protein purification (see Fig. 1) and an exchange of zinc bound to the active site of the protein by nickel is avoided (2). At the same time, non-tagged zinc-finger proteins can be purified using zinc IMAC matrices, simply by their affinity to this metal (1).

Features

Usage Specific binding of his tagged and zinc finger proteins
Specifity Affinity to his tagged and zinc finger proteins
Binding capacity Depending on the application
Filling quantity Delivered as a 25 % suspension
Bead size 30 μm
Metal Ion capacity >12 µeqv Zn2+/mL.
Bead Ligand Zn-NTA

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
metal ions affinity to his tag
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Literature references
1. Vorácková, I. et al. Purification of proteins containing zinc finger domains using immobilized metal ion affinity chromatography. Protein Expr. Purif. (2011) 79(1):88-95.
2. Block et. al. Immobilized-metal affinity chromatography (IMAC) a review. Methods Enzymol. (2009), 463:439-73.
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PureCube Zn-NTA MagBeads PureCube Zn-NTA MagBeads
Article number: 31501-Zn
Zn-NTA MagBeads
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PureCube Zn-NTA MagBeads PureCube Zn-NTA MagBeads
1 ml 25% NTA magnetic beads, loaded with zinc (II) chloride
Article number: 31501-Zn
Sales price: From €66.00 *
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