The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. PureCube StrepTactin MagBeads are ferrimagnetic agarose beads coupled to the Strep-Tactin® protein; a useful system for protein purification and pull-down experiments to determine protein-protein interactions. PureCube HiCap StrepTactin MagBeads are delivered as a 5% suspension. For detection of strep-tagged proteins in Western Blots specific antibodies and antibody conjugates
PureCube HiCap StrepTactin MagBeads provide:
- Binding capacity of up to 7 mg protein per ml settled beads
- purification of proteins expressed at low levels or from diluted solutions
- optimal conditions for pull-down experiments
- low unspecific binding
- HiCap StrepTactin Agarose also available
StrepTactin® and Strep-tag® are trademarks of IBA GmbH. PureCube StrepTactin protein is manufactured by IBA GmbH under German Patent Application No. 42 37 113.9 entitled "Fusion peptides with binding activity for streptavidin"