Nanodisc products


Nanodisc

Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. There are two options to reconstitute membrane proteins into nanodiscs:

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a His-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

Different nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own destinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed in figure 1, we also offer DIBMA based synthetic Nanodiscs here.
Nanodisc differences Titleslide
 
Size:
 
MSP1D1
MSP1D1
MSP3D1
MSP2N2
 
Species:
 
Human
Mouse
Rat
Alpaca
 
Tags:
 
MSP His tagged
Untagged MSP
Biotin Labled Nanodisc
Untagged Nanodisc
 
Lipids:
 
POPC
DMPC
DMPG
Request
 
Mutations:
 
D73C
Unmutated
 
Download the nanodisc feature table.

What our customers say:

Nanodisc Janssen Testimonial
Cube Biotech ProductUsage
Lyophilized membrane scaffold proteins for reconstitution of purified proteins
Assembled nanodiscs For cell-free reactions
Assembly kits Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein Contact us! We will help you with your project.
 
Nanodisc Products
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Synthetic Nanodisc Screening Kit, (8x2x50 mg) Synthetic Nanodisc Screening Kit, (8x2x50 mg)
Article number: 18290
Sales price: €320.00 *
MSP2N2, lyophilized protein MSP2N2, lyophilized protein
Membrane scaffold protein (MSP) 2N2, His-tag removed by proteolytic digest. 2 mg lyophilized protein
Article number: 26182
Sales price: From €312.00 *
DIBMA 10, HEPES DIBMA 10, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10x50mg | Buffered in HEPES. | MW=10.000 g/mol
Article number: 18001
Sales price: From €185.00 *
Nanodisc MSP1E3D1-His DMPC (50 µL) Nanodisc MSP1E3D1-His DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.
Article number: 26351
Sales price: €424.00 *
MSP1E3D1-His, lyophilized protein MSP1E3D1-His, lyophilized protein
Membrane scaffold protein 1 E3D1, N-terminal 6xHis-tagged, 2 mg lyophilized protein  
Article number: 26152
Sales price: From €131.00 *
SMALP 30010S SMALP 30010S
Our SMALP polymer products are made to provide an easy way to stabilize and solubilize membrane proteins.
Article number: 18210
Sales price: From €185.00 *
MSP1D1-His, lyophilized protein MSP1D1-His, lyophilized protein
2 mg lyophilized membrane scaffold 1D1 protein, His-tagged.
Article number: 26112
Sales price: From €131.00 *
Nanodisc Assembly Kit MSP1D1-His_POPC Nanodisc Assembly Kit MSP1D1-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 3.58 mg...
Article number: 26213
Sales price: €253.00 *
DIBMA 10, TRIS DIBMA 10, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=10.000 g/mol
Article number: 18004
Sales price: From €185.00 *
PureCube DIBMA Glucosamine, 1 g, HEPES DIBMA Glucosamine, HEPES
Cube Biotech offers DIBMA-Glucosamine for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18041
Sales price: From €203.00 *
PureCube DIBMA Glycerol, 1 g, HEPES DIBMA Glycerol, HEPES
Cube Biotech offers DIBMA-Glycerol in HEPES ► reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18051
Sales price: From €203.00 *
MSP1D1, lyophilized protein MSP1D1, lyophilized protein
Membrane scaffold protein (MSP)1D1, His-tag removed by proteolytic digest. 2 mg lyophilized protein
Article number: 26132
Sales price: From €251.00 *
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