Nanodisc products


Nanodisc

Nanodiscs provide an exciting alternative for the stabilization of membrane proteins. Cube Biotech offers everything you need to get started with this novel technology. There are two options to reconstitute membrane proteins into nanodiscs:

For the reconstitution of membrane proteins that are already isolated and solubilized in detergent, we recommend to use membrane scaffold proteins (MSPs) in combination with phospholipids. A variety of membrane scaffold proteins (MSPs) are available that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a His-tag, we recommend to use untagged MSP proteins. For use in cell-free expression reactions, empty nanodiscs are recommended. They can be assembled using our Nanodisc assembly kits, or purchased ready to use. Note that these kits only provide small amounts of nanodisc that are suitable for cell-free expression, and not recommended for direct reconstitution of already purified proteins.

Different nanodisc features in detail

There are several different components that a nanodisc is made from. Each with its own destinct role for the functionality of the MSP nanodisc. The following figure gives an overview of every possible composition and modification of MSP nanodiscs that we offer. In addition to the features listed in figure 1, we also offer DIBMA based synthetic Nanodiscs here.
Nanodisc differences Titleslide
 
Size:
 
MSP1D1
MSP1D1
MSP3D1
MSP2N2
 
Species:
 
Human
Mouse
Rat
Alpaca
 
Tags:
 
MSP His tagged
Untagged MSP
Biotin Labled Nanodisc
Untagged Nanodisc
 
Lipids:
 
POPC
DMPC
DMPG
Request
 
Mutations:
 
D73C
Unmutated
 
Download the nanodisc feature table.

What our customers say:

Nanodisc Janssen Testimonial
Cube Biotech ProductUsage
Lyophilized membrane scaffold proteins for reconstitution of purified proteins
Assembled nanodiscs For cell-free reactions
Assembly kits Prepare your own nanodiscs for cell-free reactions
Full nanodisc service from gene to protein Contact us! We will help you with your project.
 
Top seller
DIBMA 10, HEPES DIBMA 10, HEPES
Article number: 18001
SMALP 140-I SMALP 140-I
Article number: 18230
PureCube DIBMA Glucosamine, 1 g, HEPES DIBMA Glucosamine, HEPES
Article number: 18041
SMALP 200 SMALP 200
Article number: 18210
Nanodisc Products
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Nanodisc mouse MSP2N2-His_DMPC (50 µl) Nanodisc mouse MSP2N2-His_DMPC (50 µl)
Article number: 26771
Sales price: €557.00 *
Nanodisc mouse MSP2N2-His_POPC (50 µl) Nanodisc mouse MSP2N2-His_POPC (50 µl)
With nanodiscs you can stabilize your protein of interest.
Article number: 26773
Sales price: €557.00 *
Nanodisc mouse MSP2N2-His_DMPG (50 µl) Nanodisc mouse MSP2N2-His_DMPG (50 µl)
Assembled Nanodisc for protein solubilization in cell free systems.
Article number: 26775
Sales price: €557.00 *
DIBMA Screening Kit, HEPES (4x3x50 mg) DIBMA Screening Kit, HEPES (4x3x50 mg)
Unsure which DIBMA is best suited for your membrane protein experiments? Use our DIBMA HEPES Screening Set to find out.
Article number: 18101
Sales price: €174.25 * €205.00 *
DIBMA 12, TRIS DIBMA 12, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18014
Sales price: From €157.25 * €185.00 *
DIBMA 10, TRIS DIBMA 10, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=10.000 g/mol
Article number: 18004
Sales price: From €157.25 * €185.00 *
PureCube DIBMA Glucosamine, 1 g, TRIS DIBMA Glucosamine, TRIS
Cube Biotech offers DIBMA-Glucosamine for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18044
Sales price: From €172.55 * €203.00 *
PureCube DIBMA Glycerol, 1 g, TRIS DIBMA Glycerol, TRIS
Cube Biotech offers DIBMA-Glycerol for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18054
Sales price: From €172.55 * €203.00 *
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