Assembled nanodiscs Biotin-labeled


Nanodisc

Assembled nanodiscs, biotin-labeled

Our assembled nanodiscs are available prepared with a mixture of standard phospholipids (DMPC or POPC) and headgroup-biotinylated phosphoethanolamine (PE). The biotin label can be used for immobilization or detection of the nanodisc via Streptavidin. Biotin-labeled assembled nanodiscs are conveniently aliquoted in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Protein activity may depend on the lipid composition of the assembled nanodisc. Please contact us for other combinations of membrane scaffold proteins and lipid mixtures.
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

 

Features

Purpose Forms nanodisc structures in combination with phospholipids
Specifity Stabilization of membrane proteins
Characteristics MSP1D1, MSP1E3D1 or MSP2N2 wild-type protein with DMPC, POPC or DMPG
Resulting nanodisc diameter: ca. 7-17 nm
Purity > 90 %
Component Assembled nanodisc with DMPC, POPC or DMPG
Required equipment
 
  • Micropipettor and Micropipetting tips
  • Gel filtration column
  • FPLC instrument with integrated UV detector and fraction collector
  • Magnetic stirrer
  • Centrifuge for 2 mL microtubes
  • 2 mL microtubes
  • Palmitoyl-oleoyl-phosphatidylcholine (POPC)
  • Dimyristoyl-glycero-phosphocholine (DMPC) or other suitable phospholipid or phospholipid mixture
  • Sodium cholate
  • Biobeads SM-2
  • Single use syringe
  • End-over-end shaker
  • Single use needle
  • SDS PAGE reagents and equipment
  • Protein concentrator
  • 0.45 micron filter
  • Optional: Western Blot reagents and equipment and PentaHis Antibody
Nanodisc

Assembled nanodiscs, biotin-labeled

Our assembled nanodiscs are available prepared with a mixture of standard phospholipids (DMPC or POPC) and headgroup-biotinylated phosphoethanolamine (PE). The biotin label can be used for immobilization or detection of the nanodisc via Streptavidin. Biotin-labeled assembled nanodiscs are conveniently aliquoted in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Protein activity may depend on the lipid composition of the assembled nanodisc. Please contact us for other combinations of membrane scaffold proteins and lipid mixtures.
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

 

Features

Purpose Forms nanodisc structures in combination with phospholipids
Specifity Stabilization of membrane proteins
Characteristics MSP1D1, MSP1E3D1 or MSP2N2 wild-type protein with DMPC, POPC or DMPG
Resulting nanodisc diameter: ca. 7-17 nm
Purity > 90 %
Component Assembled nanodisc with DMPC, POPC or DMPG
Required equipment
 
  • Micropipettor and Micropipetting tips
  • Gel filtration column
  • FPLC instrument with integrated UV detector and fraction collector
  • Magnetic stirrer
  • Centrifuge for 2 mL microtubes
  • 2 mL microtubes
  • Palmitoyl-oleoyl-phosphatidylcholine (POPC)
  • Dimyristoyl-glycero-phosphocholine (DMPC) or other suitable phospholipid or phospholipid mixture
  • Sodium cholate
  • Biobeads SM-2
  • Single use syringe
  • End-over-end shaker
  • Single use needle
  • SDS PAGE reagents and equipment
  • Protein concentrator
  • 0.45 micron filter
  • Optional: Western Blot reagents and equipment and PentaHis Antibody
Assembled nanodiscs Biotin-labeled
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Nanodisc MSP1D1-His_DMPC_Biotinyl_PE (50 µl) Nanodisc MSP1D1-His_DMPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1-His protein and DMPC + 10% biotinylated PE lipids. For use in cell-free reactions. Shipped on dry ice - additional freight cost may apply.
Article number: 26411
Sales price: €567.00 *
Nanodisc MSP1D1-His_POPC_Biotinyl_PE (50 µl) Nanodisc MSP1D1-His_POPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1-His protein and POPC + 10% biotinylated PE lipids. For use in cell-free reactions. Shipped on dry ice - additional freight cost may apply.
Article number: 26413
Sales price: €567.00 *
Nanodisc MSP1D1 dH5-His_DMPC_Biotinyl_PE (50 µl) Nanodisc MSP1D1 dH5-His_DMPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1deltaH5-His protein and DMPC + 10% biotinylated PE lipids. For use in cell-free reactions. Product is shipped on dry ice - additional freight charges apply.
Article number: 26421
Sales price: €567.00 *
Nanodisc MSP1D1 dH5-His_POPC_Biotinyl_PE (50 µl) Nanodisc MSP1D1 dH5-His_POPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1deltaH5-His protein and POPC + 10% biotinylated PE lipids. For use in cell-free reactions. Shipped on dry ice - additional freight cost may apply.
Article number: 26423
Sales price: €567.00 *
Nanodisc MSP1E3D1-His_DMPC_Biotinyl_PE (50 µl) Nanodisc MSP1E3D1-His_DMPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1-His protein and DMPC + 10% biotinylated PE lipids. For use in cell-free reactions. Shipped on dry ice - additional freight cost may apply.
Article number: 26451
Sales price: €567.00 *
Nanodisc MSP1E3D1-His_POPC_Biotinyl_PE (50 µl) Nanodisc MSP1E3D1-His_POPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1-His protein and POPC + 10% biotinylated PE lipids. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26453
Sales price: €567.00 *
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