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Assembled Nanodiscs Mouse/Rat


Nanodisc

Mouse/Rat assembled nanodiscs

Assembled nanodiscs can replace detergent in cell-free expression reactions. The nascent membrane protein integrates into the nanodiscs, forming a stable complex. As human scaffold proteins might interfere with certain applications such as immunization of mice or rats with human antigens, nanodiscs containing animal homologue proteins are introduced. Nanodiscs are conveniently aliquoted in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction typically range from 20-80 µM. As protein activity may depend on the lipid composition of the assembled nanodisc, it is recommended to test several lipids or lipid mixtures. Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. For the reconstitution of membrane proteins already solubilized in detergent, please refer to lyophilized mouse membrane scaffold proteins.

Why mouse or rat assembled nanodiscs?

  • Ideal in combination with E.coE.coli cell-free expression lysates
  • No interference in mouse/rat immunization experiments
  • DMPC and POPC phospholipids available
  • MSP1D1 (wild-type) and MSPE3D1 scaffold proteins
  • Biotinylation and other lipid modifications optional
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Assembled Nanodiscs Mouse/Rat
Nanodisc

Mouse/Rat assembled nanodiscs

Assembled nanodiscs can replace detergent in cell-free expression reactions. The nascent membrane protein integrates into the nanodiscs, forming a stable complex. As human scaffold proteins might interfere with certain applications such as immunization of mice or rats with human antigens, nanodiscs containing animal homologue proteins are introduced. Nanodiscs are conveniently aliquoted in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction typically range from 20-80 µM. As protein activity may depend on the lipid composition of the assembled nanodisc, it is recommended to test several lipids or lipid mixtures. Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. For the reconstitution of membrane proteins already solubilized in detergent, please refer to lyophilized mouse membrane scaffold proteins.

Why mouse or rat assembled nanodiscs?

  • Ideal in combination with E.coE.coli cell-free expression lysates
  • No interference in mouse/rat immunization experiments
  • DMPC and POPC phospholipids available
  • MSP1D1 (wild-type) and MSPE3D1 scaffold proteins
  • Biotinylation and other lipid modifications optional
Assembled Nanodiscs Mouse/Rat
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Nanodisc mouse MSP1D1-His_DMPC (50 µL) Nanodisc mouse MSP1D1-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing mouse MSP1D1-His protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26711
Sales price: €467.00 *
Nanodisc mouse MSP1D1-His_POPC (50 µL) Nanodisc mouse MSP1D1-His_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing mouse MSP1D1-His protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26713
Sales price: €467.00 *
Nanodisc mouse MSP1D1-His_DMPG (50 µL) Nanodisc mouse MSP1D1-His_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing mouse MSP1D1-His protein and DMPG. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26715
Sales price: €467.00 *
Nanodisc mouse MSP1E3D1-His_DMPC (50 µL) Nanodisc mouse MSP1E3D1-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing mouse MSP1E3D1-His protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26751
Sales price: €467.00 *
Nanodisc mouse MSP1E3D1-His_POPC (50 µL) Nanodisc mouse MSP1E3D1-His_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing mouse MSP1E3D1-His protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26753
Sales price: €467.00 *
Nanodisc mouse MSP1E3D1-His_DMPG (50 µL) Nanodisc mouse MSP1E3D1-His_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing mouse MSP1E3D1-His protein and DMPG. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26755
Sales price: €467.00 *
Nanodisc rat MSP1D1-His_DMPC (50 µL) Nanodisc rat MSP1D1-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing rat MSP1D1-His protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26911
Sales price: €467.00 *
Nanodisc rat MSP1D1-His_POPC (50 µL) Nanodisc rat MSP1D1-His_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing rat MSP1D1-His protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26913
Sales price: €467.00 *
Nanodisc rat MSP1D1-His_DMPG (50 µL) Nanodisc rat MSP1D1-His_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing rat MSP1D1-His protein and DMPG. For use in cell-free reactions. Shipped on dry ice - additional freight cost may apply.
Article number: 26915
Sales price: €467.00 *
Nanodisc rat MSP1E3D1-His_DMPC (50 µL) Nanodisc rat MSP1E3D1-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing rat MSP1E3D1-His protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26951
Sales price: €467.00 *
Nanodisc rat MSP1E3D1-His_POPC (50 µL) Nanodisc rat MSP1E3D1-His_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing rat MSP1E3D1-His protein and POPC. For use in cell-free reactions. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26953
Sales price: €467.00 *
Nanodisc rat MSP1E3D1-His_DMPG (50 µL) Nanodisc rat MSP1E3D1-His_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing rat MSP1E3D1-His protein and POPC. For use in cell-free reactions. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26955
Sales price: €467.00 *
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