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Assembled nanodiscs untagged


Assembled nanodiscs - untagged

Assembled nanodiscs are available as part of a collaboration with the Institute of Biophysics at the University of Frankfurt. They are provided as convenient aliquots in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Bacterial and eucaryotic membrane proteins can be expressed (Fig 1 and 2), activity may depend on the lipid composition of the assembled nanodisc. Alternatively, nanodiscs can be assembled from the pre-aliquoted components provided in our Nanodisc Assembly kits. To accommodate his-tagged membrane proteins, assembled nanodiscs prepared from untagged membrane scaffold proteins (MSPs) are now available.
Nanodisc products
Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.
What is cell-free protein expression?
What is cell-free protein expression?
Available assembled nanodisc variations:
Ideal in combination with E.coE.coli cell-free expression lysates
DMPC and POPC as eukaryotic phospholipids
DMPG as prokaryotic phospholipid
MSP1D1 (wild-type), MSP1D1dH5, MSP2N2 and MSPE3D1 scaffold proteins, both his-tagged and untagged
Biotinylated nanodiscs and other lipid modifications available
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Assembled nanodiscs untagged

Assembled nanodiscs - untagged

Assembled nanodiscs are available as part of a collaboration with the Institute of Biophysics at the University of Frankfurt. They are provided as convenient aliquots in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Bacterial and eucaryotic membrane proteins can be expressed (Fig 1 and 2), activity may depend on the lipid composition of the assembled nanodisc. Alternatively, nanodiscs can be assembled from the pre-aliquoted components provided in our Nanodisc Assembly kits. To accommodate his-tagged membrane proteins, assembled nanodiscs prepared from untagged membrane scaffold proteins (MSPs) are now available.
Nanodisc products
Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.
What is cell-free protein expression?
What is cell-free protein expression?
Available assembled nanodisc variations:
Ideal in combination with E.coE.coli cell-free expression lysates
DMPC and POPC as eukaryotic phospholipids
DMPG as prokaryotic phospholipid
MSP1D1 (wild-type), MSP1D1dH5, MSP2N2 and MSPE3D1 scaffold proteins, both his-tagged and untagged
Biotinylated nanodiscs and other lipid modifications available
Assembled nanodiscs untagged
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Nanodisc MSP1D1_DMPC (50 µL) Nanodisc MSP1D1_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26331
Sales price: €526.00 *
Nanodisc MSP1D1_POPC (50 µL) Nanodisc MSP1D1_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26333
Sales price: €526.00 *
Nanodisc MSP1D1_DMPG (50 µL) Nanodisc MSP1D1_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 protein and DMPG. FFor use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26335
Sales price: €526.00 *
Nanodisc MSP1D1 dH5_DMPC (50 µL) Nanodisc MSP1D1 dH5_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 deltaH5 protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26341
Sales price: €526.00 *
Nanodisc MSP1D1 dH5_POPC (50 µL) Nanodisc MSP1D1 dH5_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 deltaH5 protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26343
Sales price: €526.00 *
Nanodisc MSP1D1 dH5_DMPG (50 µL) Nanodisc MSP1D1 dH5_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1 deltaH5 protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26345
Sales price: €526.00 *
Nanodisc MSP1E3D1-His_DMPC (50 µL) Nanodisc MSP1E3D1-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.
Article number: 26351
Sales price: €402.00 *
Nanodisc MSP1E3D1_DMPC (50 µL) Nanodisc MSP1E3D1_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1 protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26361
Sales price: €526.00 *
Nanodisc MSP1E3D1_POPC (50 µL) Nanodisc MSP1E3D1_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1 protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26363
Sales price: €526.00 *
Nanodisc MSP1E3D1_DMPG (50 µL) Nanodisc MSP1E3D1_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1E3D1 protein and DMPG. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26365
Sales price: €526.00 *
Nanodisc MSP2N2-His_DMPC (50 µL) Nanodisc MSP2N2-His_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP2N2-His protein and DMPC. For use in cell-free reactions only. Product is shipped on dry ice - additional freight charges apply.
Article number: 26371
Sales price: €493.00 *
Nanodisc MSP2N2_DMPC (50 µL) Nanodisc MSP2N2_DMPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP2N2 protein and DMPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26381
Sales price: €606.00 *
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