Assembled nanodiscs - untagged
Assembled nanodiscs are available as part of a collaboration with the Institute of Biophysics at the University of Frankfurt. They are provided as convenient aliquots in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Bacterial and eucaryotic membrane proteins can be expressed (Fig 1 and 2), activity may depend on the lipid composition of the assembled nanodisc. Alternatively, nanodiscs can be assembled from the pre-aliquoted components provided in our Nanodisc Assembly kits
. To accommodate his-tagged membrane proteins, assembled nanodiscs prepared from untagged membrane scaffold proteins (MSPs)
are now available.
Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.
Available assembled nanodisc variations:
DMPC and POPC as eukaryotic phospholipids
DMPG as prokaryotic phospholipid
MSP1D1 (wild-type), MSP1D1dH5, MSP2N2 and MSPE3D1 scaffold proteins, both his-tagged