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Assembled nanodiscs untagged


Nanodisc

Assembled nanodiscs - untagged

Assembled nanodiscs are available as part of a collaboration with the Institute of Biophysics at the University of Frankfurt. They are provided as convenient aliquots in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Bacterial and eucaryotic membrane proteins can be expressed (Fig 1 and 2), activity may depend on the lipid composition of the assembled nanodisc. Alternatively, nanodiscs can be assembled from the pre-aliquoted components provided in our Nanodisc Assembly kits. To accommodate his-tagged membrane proteins, assembled nanodiscs prepared from untagged membrane scaffold proteins (MSPs) are now available.
Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

Available assembled nanodisc variations:

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Assembled nanodiscs untagged
Nanodisc

Assembled nanodiscs - untagged

Assembled nanodiscs are available as part of a collaboration with the Institute of Biophysics at the University of Frankfurt. They are provided as convenient aliquots in 50 µL solutions of 0.5 mM concentration. End concentrations in the cell-free reaction range from 20-80 µM. Bacterial and eucaryotic membrane proteins can be expressed (Fig 1 and 2), activity may depend on the lipid composition of the assembled nanodisc. Alternatively, nanodiscs can be assembled from the pre-aliquoted components provided in our Nanodisc Assembly kits. To accommodate his-tagged membrane proteins, assembled nanodiscs prepared from untagged membrane scaffold proteins (MSPs) are now available.
Please note that assembled nanodiscs are intended for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

Available assembled nanodisc variations:

Assembled nanodiscs untagged
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Nanodisc MSP2N2_POPC (50 µL) Nanodisc MSP2N2_POPC (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP2N2 protein and POPC. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26383
Sales price: €606.00 *
Nanodisc MSP2N2_DMPG (50 µL) Nanodisc MSP2N2_DMPG (50 µL)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP2N2 protein and DMPG. For use in cell-free reactions only. Shipped on dry ice - additional freight cost apply.
Article number: 26385
Sales price: €606.00 *
Nanodisc MSP1D1-His_DMPC_Biotinyl_PE (50 µl) Nanodisc MSP1D1-His_DMPC_Biotinyl_PE (50 µl)
50 microliter pre-assembled nanodisc, 0.5 mM concentrated, containing MSP1D1-His protein and DMPC + 10% biotinylated PE lipids. For use in cell-free reactions. Shipped on dry ice - additional freight cost may apply.
Article number: 26411
Sales price: €567.00 *
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