Nanodisc Assembly kits
Nanodisc Assembly kits provide convenient aliquots and protocols for the generation of empty nanodiscs. These are recommended for use with cell-free protein expression reactions, where the nascent membrane proteins can insert directly into the nanodisc without prior detergent extraction. A variety of Nanodisc Assembly kits with different combinations of membrane scaffold proteins (MSPs) and phospholipids are available, that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. Alternatively, already assembled nanodiscs
are available. Please note that for the reconstitution of already purified membrane proteins, membrane scaffold proteins
are recommended. They can be used in combination with different amounts and types of detergents and phospholipids.
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.
Why Nanodisc Assembly kits?
Ready to use aliquots of MSPs, phospholipids, and sodium cholate
Detailed protocols and expert support provided