Nanodisc assembly kits | Cube Biotech

Nanodisc Assembly kits


Nanodisc

Nanodisc Assembly kits

Nanodisc Assembly kits provide convenient aliquots and protocols for the generation of empty nanodiscs. These are recommended for use with cell-free protein expression reactions, where the nascent membrane proteins can insert directly into the nanodisc without prior detergent extraction. A variety of Nanodisc Assembly kits with different combinations of membrane scaffold proteins (MSPs) and phospholipids are available, that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. Alternatively, already assembled nanodiscs are available. Please note that for the reconstitution of already purified membrane proteins, membrane scaffold proteins are recommended. They can be used in combination with different amounts and types of detergents and phospholipids.
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

Why Nanodisc Assembly kits?

  • Ready to use aliquots of MSPs, phospholipids, and sodium cholate
  • Ideal for supplementing cell-free expression reactions
  • Detailed protocols and expert support provided

Features

Purpose Forms nanodisc structures in combination with phospholipids
Specifity Stabilization of membrane proteins
Characteristics MSP1D1, MSP1E3D1 or MSP2N2 wild-type protein with DMPC, POPC or DMPG
Resulting nanodisc diameter: ca. 7-17 nm
Purity > 90 %
Component Assembled nanodisc with DMPC, POPC or DMPG and sodium cholate
Required equipment
 
  • Micropipettor and Micropipetting tips
  • Gel filtration column
  • FPLC instrument with integrated UV detector and fraction collector
  • Magnetic stirrer
  • Centrifuge for 2 mL microtubes
  • 2 mL microtubes
  • Palmitoyl-oleoyl-phosphatidylcholine (POPC)
  • Dimyristoyl-glycero-phosphocholine (DMPC) or other suitable phospholipid or phospholipid mixture
  • Sodium cholate
  • Biobeads SM-2
  • Single use syringe
  • End-over-end shaker
  • Single use needle
  • SDS PAGE reagents and equipment
  • Protein concentrator
  • 0.45 micron filter
  • Optional: Western Blot reagents and equipment and PentaHis Antibody
Nanodisc

Nanodisc Assembly kits

Nanodisc Assembly kits provide convenient aliquots and protocols for the generation of empty nanodiscs. These are recommended for use with cell-free protein expression reactions, where the nascent membrane proteins can insert directly into the nanodisc without prior detergent extraction. A variety of Nanodisc Assembly kits with different combinations of membrane scaffold proteins (MSPs) and phospholipids are available, that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. Alternatively, already assembled nanodiscs are available. Please note that for the reconstitution of already purified membrane proteins, membrane scaffold proteins are recommended. They can be used in combination with different amounts and types of detergents and phospholipids.
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.

Why Nanodisc Assembly kits?

  • Ready to use aliquots of MSPs, phospholipids, and sodium cholate
  • Ideal for supplementing cell-free expression reactions
  • Detailed protocols and expert support provided

Features

Purpose Forms nanodisc structures in combination with phospholipids
Specifity Stabilization of membrane proteins
Characteristics MSP1D1, MSP1E3D1 or MSP2N2 wild-type protein with DMPC, POPC or DMPG
Resulting nanodisc diameter: ca. 7-17 nm
Purity > 90 %
Component Assembled nanodisc with DMPC, POPC or DMPG and sodium cholate
Required equipment
 
  • Micropipettor and Micropipetting tips
  • Gel filtration column
  • FPLC instrument with integrated UV detector and fraction collector
  • Magnetic stirrer
  • Centrifuge for 2 mL microtubes
  • 2 mL microtubes
  • Palmitoyl-oleoyl-phosphatidylcholine (POPC)
  • Dimyristoyl-glycero-phosphocholine (DMPC) or other suitable phospholipid or phospholipid mixture
  • Sodium cholate
  • Biobeads SM-2
  • Single use syringe
  • End-over-end shaker
  • Single use needle
  • SDS PAGE reagents and equipment
  • Protein concentrator
  • 0.45 micron filter
  • Optional: Western Blot reagents and equipment and PentaHis Antibody
Nanodisc Assembly kits
Close filters
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
  •  
No results were found for the filter!
Nanodisc Assembly Kit MSP1D1-His_POPC Nanodisc Assembly Kit MSP1D1-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 3.58 mg...
Article number: 26213
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1 dH5-His_POPC Nanodisc Assembly Kit MSP1D1 dH5-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, N-terminal 6xHis-tagged, 3.12 mg...
Article number: 26223
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1E3D1-His_POPC Nanodisc Assembly Kit MSP1E3D1-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1E3, N-terminal 6xHis-tagged, 4.22 mg...
Article number: 26253
Sales price: €240.00 *
Nanodisc Assembly Kit MSP2N2-His _POPC Nanodisc Assembly Kit MSP2N2-His _POPC
Assemble your own nanodiscs (diameter 17nm) using the membrane scaffolding protein MSP2N2 (human) and the phospholipid POPC.
Article number: 26273
Sales price: €300.00 *
Nanodisc Assembly Kit MSP1D1-His_DMPC Nanodisc Assembly Kit MSP1D1-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 4.65 mg...
Article number: 26211
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1 dH5-His_DMPC Nanodisc Assembly Kit MSP1D1 dH5-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, N-terminal 6xHis-tagged, 4.87 mg...
Article number: 26221
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1_DMPC Nanodisc Assembly Kit MSP1D1_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, untagged, 4.65 mg...
Article number: 26231
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1D1_POPC Nanodisc Assembly Kit MSP1D1_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, untagged, 3.58 mg...
Article number: 26233
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1D1 dH5_DMPC Nanodisc Assembly Kit MSP1D1 dH5_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, untagged, 4.87 mg...
Article number: 26241
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1D1 dH5_POPC Nanodisc Assembly Kit MSP1D1 dH5_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, untagged, 3.12 mg...
Article number: 26243
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1E3D1-His_DMPC Nanodisc Assembly Kit MSP1E3D1-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1E3, N-terminal 6xHis-tagged, 5.09 mg...
Article number: 26251
Sales price: €240.00 *
Nanodisc Assembly Kit MSP2N2-His _DMPC Nanodisc Assembly Kit MSP2N2-His _DMPC
Assemble your own nanodiscs (diameter 17nm) using the membrane scaffolding protein MSP2N2 (human) and the phospholipid DMPC.
Article number: 26271
Sales price: €300.00 *
Viewed