Nanodisc assembly kits


Nanodisc Assembly kits

Nanodisc Assembly kits provide convenient aliquots and protocols for the generation of empty nanodiscs. These are recommended for use with cell-free protein expression reactions, where the nascent membrane proteins can insert directly into the nanodisc without prior detergent extraction. A variety of Nanodisc Assembly kits with different combinations of membrane scaffold proteins (MSPs) and phospholipids are available, that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. Alternatively, already assembled nanodiscs are available. Please note that for the reconstitution of already purified membrane proteins, membrane scaffold proteins are recommended. They can be used in combination with different amounts and types of detergents and phospholipids.
Nanodisc products
What is cell-free protein expression?
What is cell-free protein expression?
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.
Why Nanodisc Assembly kits?
Ready to use aliquots of MSPs, phospholipids, and sodium cholate
Ideal for supplementing cell-free expression reactions
Detailed protocols and expert support provided
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Nanodisc assembly kits

Nanodisc Assembly kits

Nanodisc Assembly kits provide convenient aliquots and protocols for the generation of empty nanodiscs. These are recommended for use with cell-free protein expression reactions, where the nascent membrane proteins can insert directly into the nanodisc without prior detergent extraction. A variety of Nanodisc Assembly kits with different combinations of membrane scaffold proteins (MSPs) and phospholipids are available, that can be assembled to nanodiscs of different sizes to accommodate proteins with varying numbers of transmembrane domains. His-tagged MSPs allow for easy detection of the nanodisc and even immobilization on SPR chips. On the other hand, if the protein of interest itself carries a his-tag, we recommend to use untagged MSP proteins. Alternatively, already assembled nanodiscs are available. Please note that for the reconstitution of already purified membrane proteins, membrane scaffold proteins are recommended. They can be used in combination with different amounts and types of detergents and phospholipids.
Nanodisc products
What is cell-free protein expression?
What is cell-free protein expression?
Please note that assembled nanodiscs are for use in cell-free expression reactions only. They are not recommended for the reconstitution of membrane proteins already solubilized in detergent, or for the solubilization of proteins from membranes.
Why Nanodisc Assembly kits?
Ready to use aliquots of MSPs, phospholipids, and sodium cholate
Ideal for supplementing cell-free expression reactions
Detailed protocols and expert support provided
Nanodisc Assembly kits
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Nanodisc Assembly Kit MSP1D1-His_DMPC Nanodisc Assembly Kit MSP1D1-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 4.65 mg...
Article number: 26211
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1-His_POPC Nanodisc Assembly Kit MSP1D1-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 3.58 mg...
Article number: 26213
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1 dH5-His_DMPC Nanodisc Assembly Kit MSP1D1 dH5-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, N-terminal 6xHis-tagged, 4.87 mg...
Article number: 26221
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1 dH5-His_POPC Nanodisc Assembly Kit MSP1D1 dH5-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, N-terminal 6xHis-tagged, 3.12 mg...
Article number: 26223
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1D1_DMPC Nanodisc Assembly Kit MSP1D1_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, untagged, 4.65 mg...
Article number: 26231
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1D1_POPC Nanodisc Assembly Kit MSP1D1_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, untagged, 3.58 mg...
Article number: 26233
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1D1 dH5_DMPC Nanodisc Assembly Kit MSP1D1 dH5_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, untagged, 4.87 mg...
Article number: 26241
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1D1 dH5_POPC Nanodisc Assembly Kit MSP1D1 dH5_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1 delta H5 mutant, untagged, 3.12 mg...
Article number: 26243
Sales price: €458.00 *
Nanodisc Assembly Kit MSP1E3D1-His_DMPC Nanodisc Assembly Kit MSP1E3D1-His_DMPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1E3, N-terminal 6xHis-tagged, 5.09 mg...
Article number: 26251
Sales price: €240.00 *
Nanodisc Assembly Kit MSP1E3D1-His_POPC Nanodisc Assembly Kit MSP1E3D1-His_POPC
Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1E3, N-terminal 6xHis-tagged, 4.22 mg...
Article number: 26253
Sales price: €240.00 *
Nanodisc Assembly Kit MSP2N2-His _DMPC Nanodisc Assembly Kit MSP2N2-His _DMPC
Article number: 26271
Sales price: €300.00 *
Nanodisc Assembly Kit MSP2N2-His _POPC Nanodisc Assembly Kit MSP2N2-His _POPC
Article number: 26273
Sales price: €300.00 *
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