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Nanodisc assembly kit to prepare empty nanodiscs e.g. for assembly of nascent proteins in cell-free expression reactions. Kit contents: 2 mg lyophilized Membrane scaffold protein 1 D1, N-terminal 6xHis-tagged, 4.65 mg dimyristoyl-glycero-phosphocholine (DMPC), 20 mg sodium cholate. Content of kit yields about 50 microliter nanodisc suspension in a concentration of ca. 10 mg/ml.