Synthetic nanodisc products


Dibma with protein
Synthetic nanodiscs are disc shaped complexes made from synthetic polymers like DIBMA or SMAs (1,2) that are one the best ways to solubilize and stabilize a membrane protein of interest. Their development and use in the field of proteomics is one of the greatest advances pf the field. Traditionally Detergents (like SDS, n-octyl-β-d-glucopyranoside (OG), n-dodecyl-β-d-maltoside (DDM) are used for solubilization and stabilization of membrane proteins from the cell membrane. However these detergents can interfere with affect the functional properties of a membrane protein (3). Synthetic nanodiscs are mimicing the native lipid membrane by cutting out the membrane protein of interest out of the cell membrane together with its original lipid enviroment. The alternative to Synthetic Nanodiscs are the MSP-nanodiscs.
Update November 2020: We added SMA to our product assortment! With the help of Orbiscope we now offer a second options to create synthetic nanodiscs for membrane protein solubilization & stabilization!
Currently these are CubeBiotech's options for the creation of synthetic nanodiscs:

DIBMA

SMA

DIBMAFeature
Formula Weight ~12,000 g/mol or 10,000 g/mol
pH 7.5 in buffer
Structure
Dibma Structure
Fig. 1: A GST tagged protein
SMAFeature
Formula Weight 5,000-10,000 g/mol
Depending on SMA polymer
pH 4,5 - 8,2
Structure
Note that this is the default structure for most SMAs, but not all. For example SMALP 1100I is imidized.
SMA Structure
DIBMA is the perfect choice for membrane protein solubilization and subsequent stabilization without the need for detergents (4). One further advantage of DIBMA is the lack of any meaningful absorbance of the protein specific wavelength of 280 nm. Therefore quantifying a protein after treatment with DIBMA is not influenced by any absorbance signal by the polymer.
Similar to DIBMA, SMA is a synthetic polymer that can create synthetic nanodiscs for the solubilization & stabilization for membrane proteins. The key difference between the two lies in the superior solulization rate of SMA compared to DIBMA. However SMA shows noticeable absorbance at 280 nm.
SMA or DIBMA
The question if SMA or DIBMA is the best option for your protein of interest is a tricky one. Especially if you have never worked with your current membrane protein of interest before. For these cases we compiled our most prominent SMALP and DIBMA products into this practical Synthetic Nanodisc Screening Set for your convenience!
All 8 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 8 products are include in the Kit precisely can be found in the product's description.

References

  1. Knowles, Timothy J., et al. "Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer." Journal of the American Chemical Society 131.22 (2009): 7484-7485.
  2. Oluwole, Abraham Olusegun, et al. "Solubilization of Membrane Proteins into Functional Lipid‐Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer." Angewandte Chemie International Edition 56.7 (2017): 1919-1924.
  3. Seddon, Annela M., Paul Curnow, and Paula J. Booth. "Membrane proteins, lipids and detergents: not just a soap opera." Biochimica et Biophysica Acta (BBA)-Biomembranes 1666.1-2 (2004): 105-117.
  4. Oluwole, Abraham Olusegun, et al. "Formation of lipid-bilayer nanodiscs by diisobutylene/maleic acid (DIBMA) copolymer." 33.50 (2017): 14378-14388.
 
Top seller
DIBMA Screening Kit, TRIS (12x50 mg) DIBMA Screening Kit, TRIS (12x50 mg)
Article number: 18104
DIBMA Screening Kit, HEPES (12x50 mg) DIBMA Screening Kit, HEPES (12x50 mg)
Article number: 18101
Synthetic Nanodisc Screening Kit, (8x2x50 mg) Synthetic Nanodisc Screening Kit, (8x2x50 mg)
Article number: 18290
SMALP 25010S SMALP 25010S
Article number: 18200
DIBMA 12, HEPES DIBMA 12, HEPES
Article number: 18011
DIBMA 12, TRIS DIBMA 12, TRIS
Article number: 18014
DIBMA 10, TRIS DIBMA 10, TRIS
Article number: 18004
PureCube DIBMA Glucosamine, 1 g, TRIS DIBMA Glucosamine, TRIS
Article number: 18044
Synthetic nanodisc products
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DIBMA Screening Kit, TRIS (12x50 mg) DIBMA Screening Kit, TRIS (12x50 mg)
Unsure which DIBMA is best suited for your membrane protein experiments? Use our TRIS Screening Set to find out.
Article number: 18104
Sales price: €199.00 *
DIBMA Screening Kit, HEPES (12x50 mg) DIBMA Screening Kit, HEPES (12x50 mg)
Unsure which DIBMA is best suited for your membrane protein experiments? Use our DIBMA HEPES Screening Set to find out.
Article number: 18101
Sales price: €199.00 *
Synthetic Nanodisc Screening Kit, (8x2x50 mg) Synthetic Nanodisc Screening Kit, (8x2x50 mg)
Article number: 18290
Sales price: €310.00 *
SMALP 25010S SMALP 25010S
Article number: 18200
Sales price: From €179.00 *
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DIBMA 12, HEPES DIBMA 12, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18011
Sales price: From €152.15 * €179.00 *
Details
DIBMA 12, TRIS DIBMA 12, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18014
Sales price: From €152.15 * €179.00 *
Details
DIBMA 10, TRIS DIBMA 10, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=10.000 g/mol
Article number: 18004
Sales price: From €152.15 * €179.00 *
Details
PureCube DIBMA Glucosamine, 1 g, TRIS DIBMA Glucosamine, TRIS
Cube Biotech offers DIBMA-Glucosamine for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18044
Sales price: From €197.00 *
Details
PureCube DIBMA Glycerol, 1 g, TRIS DIBMA Glycerol, TRIS
Cube Biotech offers DIBMA-Glycerol for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18054
Sales price: From €197.00 *
Details
DIBMA 10, HEPES DIBMA 10, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10x50mg | Buffered in HEPES. | MW=10.000 g/mol
Article number: 18001
Sales price: From €152.15 * €179.00 *
Details
PureCube DIBMA Glucosamine, 1 g, HEPES DIBMA Glucosamine, HEPES
Cube Biotech offers DIBMA-Glucosamine for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18041
Sales price: From €197.00 *
Details
PureCube DIBMA Glycerol, 1 g, HEPES DIBMA Glycerol, HEPES
Cube Biotech offers DIBMA-Glycerol in HEPES ► reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18051
Sales price: From €197.00 *
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