AASTY Screening Kit (6x2x50 mg)

AASTY Screening Kit Contents
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Each membrane proteins has its own native lipid environment. Therefore, each lipid... more
Product information "AASTY Screening Kit (6x2x50 mg)"
Each membrane proteins has its own native lipid environment. Therefore, each lipid environment is unique to each membrane protein. And it is this lipid environment that synthetic polymers like AASTYs interact with. Therefore one needs a broader selection of polymers, since there is not one AASTY that fits each phospholipid composition.

To address this question AASTYs come in three different ratios of acrylic acid to styrene. The ratios differ in hydrophilic property of AASTY which results in different affinities to varying membrane lipid compositions.

The second feature to screen for when it comes to AASTY is the size of the resulting nanodiscs. One of AASTY's greatest features compared to SMA or DIBMA, is that its nanodiscs are quite uniformous in size. Therefore the correct fitting nanodisc size must be determined in before.

Figure 1 shows the necessity for a AASTY screen instead of using a random SMALP product.
AASTY Screening Assay Figure 1: Example of a AASTY screening assay. As you can see there are noticable differences between the performance of different AASTYs. Also the concentration of the polymer can be varied. These performances shift from membrane protein to membrane protein. Therefore, a screening assay is always recommended.

Our SMALP Screening Set consists of the following four individual products:

Feature AASTY
6-45
AASTY
11-45
AASTY
6-50
AASTY
11-50
AASTY
6-55
AASTY
11-55
Molecular Weight 5-6 kDa 10-11 kDa 5-6 kDa 10-11 kDa 5-6 kDa 10-11 kDa
pH after solving 7.5 7.5 7.5 7.5 7.5 7.5
Divalent cationic tolerance 6 mM 6 mM 6 mM 6 mM 6 mM 6 mM
Acrylic Acid : Styrene Ratio 45% to 55% 45% to 55% 50% to 50% 50% to 50% 55% to 45% 55% to 45%

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