Synthetic Nanodisc Screening Kit, (8x2x50 mg)

Finding the best performing snythetic nanodisc polymer for your respective nanodisc can be tricky. Especially if you have never worked with your current membrane protein of interest before. For these cases we at Cube Biotech compiled our most prominent SMALP and DIBMA products into this practical Synthetic Nanodisc Screening Set for your convenience!
All 8 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 8 products are include in the Kit precisely can be found in the product's description.

Figure 1 shows the necessity for a SMALP screen instead of using a random SMALP product. Note that the detergent LMNG may provide the mighest protein yield, but it has to be added to the purified protein constantly and it might interfere with the SPIKE protein's function.

Included SMALP Products

Feature	SMALP 1100I	SMALP 25010S	SMALP 30010S	SMALP 40005S
Polymer styrene-to-maleic-anhydride ratio [n:m]	1.4:1	3:1	2:1	1.4:1
Molecular weight	5,000 Da	10,000 Da	6,500 Da	5,000 Da
Form	Lyophilized powder
pH	7.5
Divalent cationic tolerance	< 100 mM	< 5 mM	< 5 mM	< 5 mM
Color	Brownish	Colorless	Yellowish	Amber
Odor	Odorless
Other noteworthy feature	is imidized with DMAPA		Most commonly used SMA

Included DIBMA Products

Feature	DIBMA 10	DIBMA 12	DIBMA Glycerol	DIBMA Glucosamine
Formula Weight	10,000 Da	12,000 Da	10,000 Da	10,000 Da
Solubility	> 10 % in H2O
Absorbance at 280 nm	< 0.3 (1 % solution)
Mg2+ Tolerance	25 mM	25 mM	>50 mM	>50 mM
Ca2+ Tolerance	starts to precipitate 50 mM	starts to precipitate at 50 mM	>50 mM	>50 mM