Synthetic Nanodisc Screening Kit, (8x2x50 mg)
- Order number: 18290
Finding the best performing snythetic nanodisc polymer for your respective nanodisc can... more
Product information "Synthetic Nanodisc Screening Kit, (8x2x50 mg)"
Finding the best performing snythetic nanodisc polymer for your respective nanodisc can be tricky. Especially if you have never worked with your current membrane protein of interest before. For these cases we at Cube Biotech compiled our most prominent SMALP and DIBMA products into this practical Synthetic Nanodisc Screening Set for your convenience!
All 8 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 8 products are include in the Kit precisely can be found in the product's description.
Figure 1 shows the necessity for a SMALP screen instead of using a random SMALP product. Note that the detergent LMNG may provide the mighest protein yield, but it has to be added to the purified protein constantly and it might interfere with the SPIKE protein's function.
All 8 products are buffered in HEPES to provide maximal comparability between the individual screening assays. Which 8 products are include in the Kit precisely can be found in the product's description.
Figure 1 shows the necessity for a SMALP screen instead of using a random SMALP product. Note that the detergent LMNG may provide the mighest protein yield, but it has to be added to the purified protein constantly and it might interfere with the SPIKE protein's function.
Figure 1: Example of a SMALP screening assay. Different SMALPs compared in after solubilisation & purification of Rho1-tagged SPIKE protein of coronavirus.Included SMALP Products
| Feature | SMALP 1100I | SMALP 25010S | SMALP 30010S | SMALP 40005S |
| Polymer styrene-to-maleic-anhydride ratio [n:m] | 1.4:1 | 3:1 | 2.3:1 | 1.4:1 |
| Molecular weight | 5,000 Da | 10,000 Da | 6,500 Da | 5,000 Da |
| Form | Lyophilized powder | |||
| pH | 7.5 | |||
| Divalent cationic tolerance | < 100 mM | < 5 mM | < 5 mM | < 5 mM |
| Color | Brownish | Colorless | Yellowish | Amber |
| Odor | Odorless | |||
| Other noteworthy feature | is imidized with DMAPA | Most commonly used SMA | ||
Included DIBMA Products
| Feature | DIBMA 10 | DIBMA 12 | DIBMA Glycerol | DIBMA Glucosamine |
| Formula Weight | 10,000 Da | 12,000 Da | 10,000 Da | 10,000 Da |
| Solubility | > 10 % in H2O | |||
| Absorbance at 280 nm | < 0.3 (1 % solution) | |||
| Mg2+ Tolerance | 25 mM | 25 mM | >50 mM | >50 mM |
| Ca2+ Tolerance | starts to precipitate 50 mM | starts to precipitate at 50 mM | >50 mM | >50 mM |
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Disclaimer
The use of styrene maleic anhydride copolymer (SMA) products for the manufacturing of styrene maleic acid copolymer - lipid particles (nanodiscs) (SMALP), and the use of SMALP, are covered by one or more of the following patents owned by Malvern Cosmeceutics Limited: US8623414, EP1890675, GB2426703, AU2006253886, JP5142898, IN261468, CN ZL200680018957.2, CA 2611144 and by the University of Birmingham US8754168.
The purchaser is licensed under those patents to use the SMA for the manufacture of SMALPs and to use SMALPs so manufactured for the purpose of research and development of proteins, including their production (including purification and solubilization), screening, testing, analysis, characterization (including structural analysis and characterization), including for the purpose of drug screening, but not for the purpose of delivery of agents to humans or other animals for therapeutic, diagnostic, prophylactic purposes, which uses are specifically prohibited.
The purchaser is licensed under those patents to use the SMA for the manufacture of SMALPs and to use SMALPs so manufactured for the purpose of research and development of proteins, including their production (including purification and solubilization), screening, testing, analysis, characterization (including structural analysis and characterization), including for the purpose of drug screening, but not for the purpose of delivery of agents to humans or other animals for therapeutic, diagnostic, prophylactic purposes, which uses are specifically prohibited.
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