SMALP 30010S

SMALP 30010S
€185.00 *

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The SMALP, among all synthetic nanodisc products has been developed for for the... more
Product information "SMALP 30010S"
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins.
After dissolving SMALP 30010P + buffer powder in dH2O, the product is ready-to-use. SMALP 30010P has a yellowish color in solution.
After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use thethe Rho1D4-tag. Cube Biotech offers the matching products for this purpose.

SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.

Features

State Lyophilized Powder, to be solved with water
Polymer styrene-to-maleic anhydride ratio 2.3:1
Molecular weight 6.500
pH after solving 7.5
Solubility 40%
Divalent cationic tolerance > 5 mM
Color Yellowish
Odor Odorless
Freeze-thaw stable? Yes
Storage 4°C for long-term
Other noteworthy feature Most commonly used SMA
Structure SMA 30010S Structure
SMA Westernblot Figure 1: Western Blot of a purified membrane protein after stabilization through SMA.
Reproduced with permission from Ms S. Nestorow, University of Birmingham.

Our protocol video that shows a full solubilization process using SMALP

 

Disclaimer

The use of styrene maleic anhydride copolymer (SMA) products for the manufacturing of styrene maleic acid copolymer - lipid particles (nanodiscs) (SMALP), and the use of SMALP, are covered by one or more of the following patents owned by Malvern Cosmeceutics Limited: US8623414, EP1890675, GB2426703, AU2006253886, JP5142898, IN261468, CN ZL200680018957.2, CA 2611144 and by the University of Birmingham US8754168.

The purchaser is licensed under those patents to use the SMA for the manufacture of SMALPs and to use SMALPs so manufactured for the purpose of research and development of proteins, including their production (including purification and solubilization), screening, testing, analysis, characterization (including structural analysis and characterization), including for the purpose of drug screening, but not for the purpose of delivery of agents to humans or other animals for therapeutic, diagnostic, prophylactic purposes, which uses are specifically prohibited.
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