SMALP 140

SMALP 140
€185.00 *

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  • 18220
Note:  On October 1st 2021 this product was renamed from "SMALP 40005S" to "SMALP... more
Product information "SMALP 140"
Note: On October 1st 2021 this product was renamed from "SMALP 40005S" to "SMALP 140". Nothing else has changed. More on this renaming can be found here:
The synthethic polymer SMALP 140, among our other synthetic nanodisc products has been developed for for the solubilization & subsequent purification of membrane proteins from all kinds of cell membranes. SMALP products do this by using the phospholipids around the membrane proteins and form them into a disc-shaped structure in which the membrane proteins are centered.
SMALP 140 + its corresponding buffer are shipped in a lyophilized state, so they have to be dissolved in dH2O. Then the product is ready-to-use. SMALP 140 has a amber color in solution.
We recommend using affinity chromatography for the follow-up purification of the membrane protein of interest. The Rho1D4-tag has proven to be especially usefull for membrane protein purification. Cube Biotech offers the matching products for this purpose.

SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between DIBMA and SMA, we also offer our, that includes both our SMA and DIBMA products.

Features

State Lyophilized Powder, to be solved with water
Polymer styrene-to-maleic anhydride ratio 1.4:1
Molecular weight 5.000
pH after solving 7.5
Solubility 45%
Divalent cationic tolerance > 5 mM
Color Amber
Odor Odorless
Freeze-thaw stable? Yes
Storage 4°C for long-term
Structure SMA 140 Structure
Western Blot using SMALPS Figure 1: Western Blot of a purified membrane protein after stabilization through SMA.
Reproduced with permission from Ms S. Nestorow, University of Birmingham.

Our protocol video that shows a full solubilization process using SMALP

 

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