SMALP 40005S

SMALP 40005S
€179.00 *

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The synthethic polymer SMALP 40005S, among our other synthetic nanodisc products has been... more
Product information "SMALP 40005S"
The synthethic polymer SMALP 40005S, among our other synthetic nanodisc products has been developed for for the solubilization & subsequent purification of membrane proteins from all kinds of cell membranes. SMALP products do this by using the phospholipids around the membrane proteins and form them into a disc-shaped structure in which the membrane proteins are centered.
SMALP 40005S + its corresponding buffer are shipped in a lyophilized state, so they have to be dissolved in dH2O. Then the product is ready-to-use. SMALP 40005S has a amber color in solution.
We recommend using affinity chromatography for the follow-up purification of the membrane protein of interest. The Rho1D4-tag has proven to be especially usefull for membrane protein purification. Cube Biotech offers the matching products for this purpose.

SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between DIBMA and SMA, we also offer our, that includes both our SMA and DIBMA products.

Features

State Lyophilized Powder, to be solved with water
Polymer styrene-to-maleic anhydride ratio 1.4:1
Molecular weight 5.000
pH after solving 7.5
Solulbility 45%
Divalent cationic tolerance > 5 mM
Color Amber
Odor Odorless
Freeze-thaw stable? Yes
Storage 4°C for long-term
Structure SMA 40005S Structure
Western Blot using SMALPS Figure 1: Western Blot of a purified membrane protein after stabilization through SMA.
Reproduced with permission from Ms S. Nestorow, University of Birmingham.

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Disclaimer

The use of styrene maleic anhydride copolymer (SMA) products for the manufacturing of styrene maleic acid copolymer - lipid particles (nanodiscs) (SMALP), and the use of SMALP, are covered by one or more of the following patents owned by Malvern Cosmeceutics Limited: US8623414, EP1890675, GB2426703, AU2006253886, JP5142898, IN261468, CN ZL200680018957.2, CA 2611144 and by the University of Birmingham US8754168.

The purchaser is licensed under those patents to use the SMA for the manufacture of SMALPs and to use SMALPs so manufactured for the purpose of research and development of proteins, including their production (including purification and solubilization), screening, testing, analysis, characterization (including structural analysis and characterization), including for the purpose of drug screening, but not for the purpose of delivery of agents to humans or other animals for therapeutic, diagnostic prophylactic purposes, which uses are specifically prohibited.
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