SMALP 140-I

SMALP 140-I
€209.00 *

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  • 18230
Note:  On October 1st 2021 this product was renamed from "SMALP 1100I" to "SMALP... more
Product information "SMALP 140-I"
Note: On October 1st 2021 this product was renamed from "SMALP 1100I" to "SMALP 140-I". Nothing else has changed. More on this renaming can be found here:
The SMALP 140-I, our other SMALP products has been developed for for the solubilization of membrane proteins. SMALP 140-I is special among other SMAs as it contains a maleimide function and therefore its standard structure derives from standard SMAs.
After dissolving buffer powder + SMALP 140-I in dH2O, the product is ready-to-use. SMALP 140-I has a brownish color in solution.
After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. We recommed to use the Rho1D4-tag for affinity chromatography of membrane proteins. Cube Biotech offers the matching products for this purpose.

SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set.
Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.

Features

State Lyophilized Powder, to be solved with water
Polymer styrene-to-maleic anhydride ratio 1.4:1
Molecular weight 5.000
pH after solving 7.5
Solubility 38%
Divalent cationic tolerance > 100 mM
Color Yellowish
Odor Odorless
Freeze-thaw stable? Yes
Storage 4°C for long-term
Other noteworthy feature Is imidized with DMAPA
Structure
Base Polymer
SMALP 140-I Structure
Western Blot using SMALP 140-I Figure 1: Western Blot of a purified membrane protein after stabilization through SMA.
Reproduced with permission from Ms S. Nestorow, University of Birmingham.

Our protocol video that shows a full solubilization process using SMALP

 

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