Synthetic Polymers


Dibma with protein
Synthetic nanodiscs are disc shaped complexes made from synthetic polymers like DIBMA or SMAs (1,2) that are one the best ways to solubilize and stabilize a membrane protein of interest. Their development and use in the field of proteomics is one of the greatest advances pf the field. Traditionally Detergents (like SDS, n-octyl-β-d-glucopyranoside (OG), n-dodecyl-β-d-maltoside (DDM) are used for solubilization and stabilization of membrane proteins from the cell membrane. However these detergents can interfere with affect the functional properties of a membrane protein (3). Synthetic nanodiscs are mimicing the native lipid membrane by cutting out the membrane protein of interest out of the cell membrane together with its original lipid enviroment. The alternative to Synthetic Nanodiscs are the MSP-nanodiscs.
Therefore the use of DIBMA is a direct way to extract membrane proteins from cells without an intermediate step of detergent solubilization (4). Synthetic polymers have to carry a styrene or maleic acid group himself to solubilize proteins.

Our go to option for the creation of synthetic nanodiscs is DIBMA. Click here to see are great selection of DIBMA variants for your specific experiment setup.


References

  1. Knowles, Timothy J., et al. "Membrane proteins solubilized intact in lipid containing nanoparticles bounded by styrene maleic acid copolymer." Journal of the American Chemical Society 131.22 (2009): 7484-7485.
  2. Oluwole, Abraham Olusegun, et al. "Solubilization of Membrane Proteins into Functional Lipid‐Bilayer Nanodiscs Using a Diisobutylene/Maleic Acid Copolymer." Angewandte Chemie International Edition 56.7 (2017): 1919-1924.
  3. Seddon, Annela M., Paul Curnow, and Paula J. Booth. "Membrane proteins, lipids and detergents: not just a soap opera." Biochimica et Biophysica Acta (BBA)-Biomembranes 1666.1-2 (2004): 105-117.
  4. Oluwole, Abraham Olusegun, et al. "Formation of lipid-bilayer nanodiscs by diisobutylene/maleic acid (DIBMA) copolymer." 33.50 (2017): 14378-14388.
 
Synthetic Polymers
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PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS PureCube DIBMA 12, Screening Kit, 10x50 mg, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10X50mg | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18014
Sales price: €179.00 *
PureCube DIBMA 12, HEPES PureCube DIBMA 12, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18012
Sales price: From €281.00 *
PureCube DIBMA Glycerol, 1 g, HEPES PureCube DIBMA Glycerol, HEPES
Cube Biotech offers DIBMA-Glycerol in HEPES ► reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18052
Sales price: From €309.00 *
PureCube DIBMA Mix & Match, Screening Kit, 12x50 mg, TRIS PureCube DIBMA Mix & Match, Screening Kit,...
Unsure which DIBMA size is best suited for your membrane protein experiments? Use our Mix & Match TRIS Kits to find out.
Article number: 18104
Sales price: €199.00 *
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PureCube DIBMA 10, Screening Kit, 10x50 mg, HEPES PureCube DIBMA 10, Screening Kit, 10x50 mg, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10X50mg | Buffered in HEPES. | MW=10.000 g/mol
Article number: 18001
Sales price: €179.00 *
PureCube DIBMA 10, Screening Kit, 10x50 mg, TRIS PureCube DIBMA 10, Screening Kit, 10x50 mg, TRIS
DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10x50mg | Buffered in TRIS. | MW=10.000 g/mol
Article number: 18004
Sales price: €179.00 *
PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES PureCube DIBMA 12, Screening Kit, 10x50 mg, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10X50mg | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18011
Sales price: €179.00 *
PureCube DIBMA 10, HEPES PureCube DIBMA 10, HEPES
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount = 10x50mg | Buffered in HEPES. | MW=10.000 g/mol
Article number: 18002
Sales price: From €281.00 *
PureCube DIBMA 10, TRIS PureCube DIBMA 10, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=10.000 g/mol
Article number: 18005
Sales price: From €281.00 *
PureCube DIBMA 12, TRIS PureCube DIBMA 12, TRIS
PureCube DIBMA by Cube-Biotech. For protein solubilization. | Amount=1 gramm | Buffered in TRIS. | MW=12.000 g/mol
Article number: 18015
Sales price: From €281.00 *
PureCube DIBMA Glucosamine, Screening Kit, 10x50 mg, HEPES PureCube DIBMA Glucosamine, Screening Kit,...
Our DIBMA-Glucosamine in a practical screening Kit. Identify the amount of DIBMA-Glucosamine that best suits your assays.
Article number: 18041
Sales price: €197.00 *
PureCube DIBMA Glucosamine, 1 g, HEPES PureCube DIBMA Glucosamine, HEPES
Cube Biotech offers DIBMA-Glucosamine for reduced net charge of the polymer. Higher ion tollerance for your experiments.
Article number: 18042
Sales price: From €309.00 *
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