Cube Affinity tag antibodies | Cube Biotech

Affinity tag antibodies


Affinity tag antibodies

Recombinant proteins carrying an affinity tag can be easily detected using tag-specific antibodies, thereby eliminating the need for protein-specific antibodies for each protein. Cube Biotech offers highly sensitive antibodies recognizing the three most commonly used affinity tags. All our antibodies are mouse monoclonal, and can be detected using the same secondary antibody, thereby facilitating processes. Sets of affinity resins and matching antibodies are available as cost-effective combinations. Table 1 shows technical and performance parameters of our three tag-specific antibodies.
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PureCube Antibodies provide:
Highly specific and sensitive detection
Recognizing His, Rho1D4, Strep, and GST tags
Suitable for Western Blot and ELISA
A perfect match to PureCube Agaroses and MagBeads
 PentaHisRho1D4GSTStrep
Epitope HHHHH TETSQVAPA Glutathione S-transferase from Schistosoma japonicum Strep-tag II
Antibody type mouse monoclonal mouse monoclonal mouse monoclonal mouse monoclonal
Detection limit in Western Blot (chemiluminescent detection) 50 pg n.d. 500 pg n.d.
Detection limit in Western Blot (chromogenic detection) 2 ng n.d. n.d. 5 ng
Recommended dilution 1:1000 1:1000 1:1000 200 ng/mL for chromogenic detection
Recommended secondary antibody e.g. goat anti mouse e.g. goat anti mouse e.g. goat anti mouse e.g. rabbit anti mouse
Packaging 100 µg lyophilized 200 µg in solution 100 µg lyophilized 100 µg lyophilized
Comment BSA-free - - HRP-conjugate also available
Table 1. Overview on technical features and performance of Cube Affinity tag antibodies.
PentaHisBlot
High sensitivity and specificity of PentaHis Antibody
The PentaHis antibody detects his-tagged proteins with high sensitivity and specificity. Background levels are low, and unambiguous signals are obtained, even at low protein concentrations. Fig. 1 shows western blot detection of his-tagged green fluorescent protein (GFP) that was applied to an SDS-PAGE in defined amounts. 100 ng of protein are clearly visualized by chemiluminescent detection. Note that detection levels are dependent on individual proteins, blot efficiency, antibody dilution, and choice of secondary antibody. Therefore, the optimal values (Table 1) are not always reached.
Fig.1: Western Blot ECL detection of purified his-tagged GFP using the Cube PentaHis antibody. SDS-PAGE was done with defined amounts. Lanes 1: 50 pg, 2: 500 pg, 3: 1 ng, 4: 100 ng, 5: 250 ng, 6: 500 ng. Dilution factors: Primary antibody: 1:2000; secondary antibody 1:5000.
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Affinity tag antibodies

Affinity tag antibodies

Recombinant proteins carrying an affinity tag can be easily detected using tag-specific antibodies, thereby eliminating the need for protein-specific antibodies for each protein. Cube Biotech offers highly sensitive antibodies recognizing the three most commonly used affinity tags. All our antibodies are mouse monoclonal, and can be detected using the same secondary antibody, thereby facilitating processes. Sets of affinity resins and matching antibodies are available as cost-effective combinations. Table 1 shows technical and performance parameters of our three tag-specific antibodies.
Spin Columns
Learn_Agaroses_CubeColors
PureCube Antibodies provide:
Highly specific and sensitive detection
Recognizing His, Rho1D4, Strep, and GST tags
Suitable for Western Blot and ELISA
A perfect match to PureCube Agaroses and MagBeads
 PentaHisRho1D4GSTStrep
Epitope HHHHH TETSQVAPA Glutathione S-transferase from Schistosoma japonicum Strep-tag II
Antibody type mouse monoclonal mouse monoclonal mouse monoclonal mouse monoclonal
Detection limit in Western Blot (chemiluminescent detection) 50 pg n.d. 500 pg n.d.
Detection limit in Western Blot (chromogenic detection) 2 ng n.d. n.d. 5 ng
Recommended dilution 1:1000 1:1000 1:1000 200 ng/mL for chromogenic detection
Recommended secondary antibody e.g. goat anti mouse e.g. goat anti mouse e.g. goat anti mouse e.g. rabbit anti mouse
Packaging 100 µg lyophilized 200 µg in solution 100 µg lyophilized 100 µg lyophilized
Comment BSA-free - - HRP-conjugate also available
Table 1. Overview on technical features and performance of Cube Affinity tag antibodies.
PentaHisBlot
High sensitivity and specificity of PentaHis Antibody
The PentaHis antibody detects his-tagged proteins with high sensitivity and specificity. Background levels are low, and unambiguous signals are obtained, even at low protein concentrations. Fig. 1 shows western blot detection of his-tagged green fluorescent protein (GFP) that was applied to an SDS-PAGE in defined amounts. 100 ng of protein are clearly visualized by chemiluminescent detection. Note that detection levels are dependent on individual proteins, blot efficiency, antibody dilution, and choice of secondary antibody. Therefore, the optimal values (Table 1) are not always reached.
Fig.1: Western Blot ECL detection of purified his-tagged GFP using the Cube PentaHis antibody. SDS-PAGE was done with defined amounts. Lanes 1: 50 pg, 2: 500 pg, 3: 1 ng, 4: 100 ng, 5: 250 ng, 6: 500 ng. Dilution factors: Primary antibody: 1:2000; secondary antibody 1:5000.
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Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
Article number: 40020
GST antibody (0.1 mg) GST antibody (0.1 mg)
Article number: 40060
Strep antibody (0.1 mg) Strep antibody (0.1 mg)
Article number: 40070
Strep antibody HRP Strep antibody HRP
Article number: 40071
Affinity tag antibodies
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Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
0.2 mg Rho1D4 antibody in solution  
Article number: 40020
Sales price: €273.00 *
PentaHis antibody, BSA-free (0.1 mg) PentaHis antibody, BSA-free (0.1 mg)
Lyophilized. Manufactured by QIAGEN.  
Article number: 40040
Sales price: €439.00 *
GST antibody (0.1 mg) GST antibody (0.1 mg)
0.1 mg GST antibody, lyophilized. Manufactured by QIAGEN.  
Article number: 40060
Sales price: €466.00 *
Strep antibody (0.1 mg) Strep antibody (0.1 mg)
0.1 mg anti-Strep tag II mouse monoclonal antibody, purified and lyophilized. Manufactured by IBA.  
Article number: 40070
Sales price: €188.00 *
Strep antibody HRP Strep antibody HRP
75 mikroliter anti-Strep tag II mouse monoclonal antibody, purified and conjugated to horseradish peroxidase. Manufactured by IBA.
Article number: 40071
Sales price: €238.00 *
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