Human iPSC-derived lipids


Human iPSC-derived lipids

Human cardiomyocyte lipids and CNS lipids are extracted from Ncardia's Cor.4U® cardiomyocytes and CNS.4U® neural cells, respectively. Both cell lines are produced by in vitro differentiation of transgenic human induced pluripotent stem cells(iPSC), generated by introducing defined transcription factors, as described by Yamanaka et al in human skin fibroblasts, using a non-viral system, and are grown in fully synthetic media. Cardiomyocytes and CNS cells are well-characterized and of high purity, and their human origin ensures optimal composition for use in human membrane protein assays.
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Human iPSC-derived lipids can restore membrane protein functionality in artificial surroundings:
Stabilization of membrane proteins in nanodiscs
SPR and other ligand-binding assays (Figure 1)
Detailed protocols for successful purification
heart lipids white_200pxH
Cardiomyocytes
The highly pure cardiomyocytes express cardiac-specific proteins, e.g., cardiac alpha-actinin and the gap junction protein connexin-43.
brain lipids_white_200pxH
CNS cells
CNS cells comprise key cell types of the human central nervous system (CNS): neurons (80-90%) and astrocytes (10-20%). Of the neurons, 90% are glutamatergic and GABAergic and 10% are dopaminergic neurons.
Icons: mariaaverburg/fotolia
TGR5 SPR_lipids_web
Figure 1: Human CNS lipids restore membrane protein functionality in surface plasmon resonance (SPR). SPR measurements were performed by immobilization of target proteins via a His tag on a Ni-NTA-functionalized chip and monitoring association/dissociation kinetics of the specific ligand Taurolithocholic acid. A: GPBAR1/TGR5 membrane protein preparation in detergent, incubated in the presence of Human CNS lipids_iPSC-derived o/n at 4°C; binding constants of 11 nM were measured, which is in accordance with published values. B: The same protein preparation was incubated at identical conditions, but without any Human CNS lipids added. No signal was obtained in this measurement.
Literature references
1. Takahashi, K et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell Nov 30;131(5):861-72
2. Axiogenesis AG Patent WO2012098260 A1, A non-viral system for generation of induced pluripotent stem (iPS) cells
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Human iPSC-derived lipids

Human iPSC-derived lipids

Human cardiomyocyte lipids and CNS lipids are extracted from Ncardia's Cor.4U® cardiomyocytes and CNS.4U® neural cells, respectively. Both cell lines are produced by in vitro differentiation of transgenic human induced pluripotent stem cells(iPSC), generated by introducing defined transcription factors, as described by Yamanaka et al in human skin fibroblasts, using a non-viral system, and are grown in fully synthetic media. Cardiomyocytes and CNS cells are well-characterized and of high purity, and their human origin ensures optimal composition for use in human membrane protein assays.
PG_Logo_PLS_150x150_RGB72
NCARDIA LOGO-RGB-OL_200px
Human iPSC-derived lipids can restore membrane protein functionality in artificial surroundings:
Stabilization of membrane proteins in nanodiscs
SPR and other ligand-binding assays (Figure 1)
Detailed protocols for successful purification
heart lipids white_200pxH
Cardiomyocytes
The highly pure cardiomyocytes express cardiac-specific proteins, e.g., cardiac alpha-actinin and the gap junction protein connexin-43.
brain lipids_white_200pxH
CNS cells
CNS cells comprise key cell types of the human central nervous system (CNS): neurons (80-90%) and astrocytes (10-20%). Of the neurons, 90% are glutamatergic and GABAergic and 10% are dopaminergic neurons.
Icons: mariaaverburg/fotolia
TGR5 SPR_lipids_web
Figure 1: Human CNS lipids restore membrane protein functionality in surface plasmon resonance (SPR). SPR measurements were performed by immobilization of target proteins via a His tag on a Ni-NTA-functionalized chip and monitoring association/dissociation kinetics of the specific ligand Taurolithocholic acid. A: GPBAR1/TGR5 membrane protein preparation in detergent, incubated in the presence of Human CNS lipids_iPSC-derived o/n at 4°C; binding constants of 11 nM were measured, which is in accordance with published values. B: The same protein preparation was incubated at identical conditions, but without any Human CNS lipids added. No signal was obtained in this measurement.
Literature references
1. Takahashi, K et al. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell Nov 30;131(5):861-72
2. Axiogenesis AG Patent WO2012098260 A1, A non-viral system for generation of induced pluripotent stem (iPS) cells
Human iPSC-derived lipids
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Human cardiomyocyte lipids_ iPSC-derived (1 mg) Human cardiomyocyte lipids_ iPSC-derived (1 mg)
Lipids extracted from human, iPSC-derived cardiomyocytes. 1 mg pellet. Shipped at 4°C.
Article number: 16521
Sales price: €490.00 *
Human CNS lipids_iPSC-derived (1 mg) Human CNS lipids_iPSC-derived (1 mg)
Lipids extracted from human, iPSC-derived neurons (80-90%) and astrocytes (10-20%). 1 mg pellet. Shipped at 4°C.
Article number: 16523
Sales price: €490.00 *
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