PureCube Compact Cartridge Glutathione

Order number: 32302

€56.00*

Ready to ship today
Quantity

Description

The pairing of an enzyme to its substrate is one of the most specific combinations in life sciences. This is why GST-tag purifications exploit this specificity to gain high levels of purity while maintaining a high protein yield. The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA TM.

The PureCube Glutathione Compact cartridge is a FPLC column pre-packed with PureCube Glutathione Agarose. Alternatively, Glutathione MagBeads are also available, if you prefer this affinity chromatography approach.

Additional support products for GST-tag purification assays are L-Glutathione (reduced), an essential compound of the elution buffer, and our GST-tag antibody for later western blot detection of your protein.
Feature - Glutathione Resin
Usage Specific binding and purification of GST-tagged proteins
Specificity Affinity to GST(Gluthathione-S-Transferase)-tagged proteins
Binding capacity >10 mg/ml
Bead Ligand L-Glutathione (reduced)
Bead size 40 μm
Matrix 7.5% cross-linked agarose
Filling quantity Delivered as a 50 % suspension
pH Stability 3-12
Required equipment & materials
  • FPLC hardware like e.g. AEKTA
  • Binding Buffer
  • Elution Buffer


Feature - Column
PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Max. Flow Rate 6 mL/min 6 mL/min
Dimensions: diameter X length (mm) 5 X 35 17 X 35
Body material Polypropylene Polypropylene
Inlet 10-32 UNF female thread 10-32 UNF female thread
Outlet 10-32 UNF female thread 10-32 UNF female thread

Lab Results

Lab funds are sadly often the limiting factor when it comes to research. So it is of utmost importance that one gets the most value from every purchase one does. The reusability of products and materials is a key word here!

Our GST-tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST-tag purification with the same beads is ensured to be of the same high quality.
Performance of PureCube Glutathione Purification beads
Figure 1: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.

FAQ

Can I get the datasheet for the PureCube Glutathione Compact Cartridge?

Why are there beads of different sizes for the same purpose?

It is a balance to strike here between protein yield (smaller beads) and other factors like higher FPLC flow rates or handling of very viscous cell media (bigger beads).

Can I regenerate & reuse your Glutathione beads?