His Affinity Cartridges


MagBeads;magnetic_beads
The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins. Cube Biotech offers high-performance PureCube IDA Agarose and NTA Agarose, and the novel INDIGO Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube Cartridges and Compact Cartridges are:

Choose your metal ion and ligand.
Ready-to use His affinity columns / cartridges with 40 µm average bead size:
Ni-NTA columns / cartridges Co-NTA columns / cartridges Ni-IDA columns / cartridges
Ready-to use His affinity agarose with 100 µm average bead size:
INDIGO Ni-Agarose 100 Ni-NTA Agarose 100 Co-NTA Agarose 100
Empty PureCube Columns / Cartridges to be filled with your agarose purification resin
  Empty Columns / Cartridges  
Metal Ions differ in their affinity & specificity to the His tag.
An important choice while working with the His Tag is the selection on the loaded metal ion to the purification resin. As most things in life sciences a trade-off scheme is occurring here. Metal Ions with a high specificity tend to have a weaker affinity to the His tag and therefore a smaller but purer protein yield in the end. On the other end of this scheme there are metal ions with a high affinity towards the His tag, but at the cost of the specificity. This leads to a higher, but less pure protein yield. Figure 1. depicts the most commonly used metal ions for his tag purification and where they belong in this scheme.
 
Affinity metal Ions
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
Example photos of our cartridges
313xx_1mL_5mL_Ni-NTA_Cartridge
Fig. 2. PureCube Ni-NTA Cartridge. 1 ml
1ml Ni-NTA_ COMPACT Cartridge
Fig. 3. PureCube Ni-NTA COMPACT Cartridge. 1 ml
Co-NTA_Cartridge
Fig. 4. PureCube Co-NTA 100 COMPACT cartridge Cartridge. 1 ml
MagBeads;magnetic_beads
The polyhistidine tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native and denaturing conditions, as well as in presence of detergents and many other additives. Taking advantage of the affinity of transition metal ions for the imidazole ring of histidine, immobilized metal affinity chromatography (IMAC) is used to purify his-tagged proteins. Cube Biotech offers high-performance PureCube IDA Agarose and NTA Agarose, and the novel INDIGO Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube Cartridges and Compact Cartridges are:

Choose your metal ion and ligand.
Ready-to use His affinity columns / cartridges with 40 µm average bead size:
Ni-NTA columns / cartridges Co-NTA columns / cartridges Ni-IDA columns / cartridges
Ready-to use His affinity agarose with 100 µm average bead size:
INDIGO Ni-Agarose 100 Ni-NTA Agarose 100 Co-NTA Agarose 100
Empty PureCube Columns / Cartridges to be filled with your agarose purification resin
  Empty Columns / Cartridges  
Metal Ions differ in their affinity & specificity to the His tag.
An important choice while working with the His Tag is the selection on the loaded metal ion to the purification resin. As most things in life sciences a trade-off scheme is occurring here. Metal Ions with a high specificity tend to have a weaker affinity to the His tag and therefore a smaller but purer protein yield in the end. On the other end of this scheme there are metal ions with a high affinity towards the His tag, but at the cost of the specificity. This leads to a higher, but less pure protein yield. Figure 1. depicts the most commonly used metal ions for his tag purification and where they belong in this scheme.
 
Affinity metal Ions
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.
Example photos of our cartridges
313xx_1mL_5mL_Ni-NTA_Cartridge
Fig. 2. PureCube Ni-NTA Cartridge. 1 ml
1ml Ni-NTA_ COMPACT Cartridge
Fig. 3. PureCube Ni-NTA COMPACT Cartridge. 1 ml
Co-NTA_Cartridge
Fig. 4. PureCube Co-NTA 100 COMPACT cartridge Cartridge. 1 ml
His Affinity Cartridges
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PureCube 100 INDIGO Ni-Cartridge PureCube 100 INDIGO Ni-Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml Agarose with 100 micron average bead size, equipped with a proprietary ligand for His affinity purification, loaded with nickel (II) sulfate For more information read...
Article number: 75301
Sales price: From €47.00 *
PureCube 100 Compact Cartridge Ni-INDIGO PureCube 100 Compact Cartridge Ni-INDIGO
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml Agarose with 100 micron average bead size, equipped with a proprietary ligand for His affinity purification, loaded with nickel (II) sulfate
Article number: 75302
Sales price: From €49.00 *
PureCube Compact Cartridge Ni-NTA PureCube Compact Cartridge Ni-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with nickel (II) sulfate
Article number: 31302
Sales price: From €33.00 *
PureCube Co-NTA Cartridge PureCube Co-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with cobalt (II) chloride
Article number: 31601
Sales price: From €36.00 *
PureCube Compact Cartridge Co-NTA PureCube Compact Cartridge Co-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with cobalt (II) chloride
Article number: 31602
Sales price: From €36.00 *
PureCube Ni-IDA Cartridge PureCube Ni-IDA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30301
Sales price: From €32.00 *
PureCube Compact Cartridge Ni-IDA PureCube Compact Cartridge Ni-IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30302
Sales price: From €32.00 *
PureCube IDA Cartridge PureCube IDA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, for loading with transition metals
Article number: 30901
Sales price: From €32.00 *
PureCube Compact Cartridge IDA PureCube Compact Cartridge IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, for loading with transition metals
Article number: 30902
Sales price: €32.00 *
PureCube Compact Cartridge IDA PureCube Compact Cartridge IDA
5 Compact Cartridges for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, for loading with transition metals
Article number: 30904
Sales price: €145.00 *
PureCube Compact Cartridge IDA PureCube Compact Cartridge IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 5 ml IDA Agarose, for loading with transition metals
Article number: 30906
Sales price: €97.00 *
PureCube Compact Cartridge IDA PureCube Compact Cartridge IDA
5 Compact Cartridges for low pressure chromatography (FPLC), packed with 5 ml IDA Agarose, for loading with transition metals
Article number: 30908
Sales price: €457.00 *
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