Co-NTA Cartridges 100


Agarose guide
The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech introduces the novel PureCube 100 Co-NTA Agarose, based on high quality crosslinked agarose with 100 µm mean diameter, providing high flow rates in batch and FPLC applications. Specificity of this transition metal for histidine stretches is typically higher than that of nickel (see Fig. 1). Due to the excellent flow properties of PureCube 100 Agarose, combined with the high capacity NTA ligand, high dynamic yield and protein purity can be achieved. Cube Biotech offers PureCube 100 Co-NTA Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube 100 Co-NTA Cartridges provide:


Co-NTA MAgBeads in detail

Affinity_specificity_MetalIons_wo_legend
Different metal ions confer different binding affinity and specificity
Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Features

Feature PureCube 100 Co-NTA Cartridge PureCube 100 Co-NTA COMPACT Cartridge
Usage Purification of his tagged proteins
Specifity Affinity to His-tagged proteins
Protein Binding capacity
20 mg (1ml cartridge)
100 mg (5ml cartridge)
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability 2-14
Other Compatibilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Bead size ~100 μm
Storage temperature 2-8 °C
Bead Ligand Co-NTA
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 6% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
Required Buffers for purification under native conditions
 
  • Native Lysis buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 10 mM, pH 8 Optional: Add 1 tablet protease inhibitor cocktail to the Lysis Buffer. Tip: Lysis Buffer contains 10 mM imidazole to prevent binding of untagged proteins. If His-tagged proteins do not bind under these conditions, reduce the imidazole concentration to 1-5 mM.
  • Native Wash buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 20 mM, pH 8
  • Native Elution buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 250- 500 mM, pH 8
Required Buffers for purification under denaturing conditions
 
  • Denaturing Lysis buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 8.0, Optional: Benzonase® nuclease (e.g. Merck Milipore, #707464)
  • Denaturing Wash buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 6.3NaH2PO4 100 mM
  • Denaturing Elution buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 4.5 Tip: If the target protein is acid-labile, elution can be performed with 250-500 mM imidazole. Note: Due to urea dissociation, adjust the pH immediately before use.
Agarose guide
The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech introduces the novel PureCube 100 Co-NTA Agarose, based on high quality crosslinked agarose with 100 µm mean diameter, providing high flow rates in batch and FPLC applications. Specificity of this transition metal for histidine stretches is typically higher than that of nickel (see Fig. 1). Due to the excellent flow properties of PureCube 100 Agarose, combined with the high capacity NTA ligand, high dynamic yield and protein purity can be achieved. Cube Biotech offers PureCube 100 Co-NTA Agarose, prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC).

PureCube 100 Co-NTA Cartridges provide:


Co-NTA MAgBeads in detail

Affinity_specificity_MetalIons_wo_legend
Different metal ions confer different binding affinity and specificity
Loading different metal ions to a resin results in differing affinity and specificity for a his-tagged protein. Generally, cobalt exhibits the higest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Fig. 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Features

Feature PureCube 100 Co-NTA Cartridge PureCube 100 Co-NTA COMPACT Cartridge
Usage Purification of his tagged proteins
Specifity Affinity to His-tagged proteins
Protein Binding capacity
20 mg (1ml cartridge)
100 mg (5ml cartridge)
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
pH stability 2-14
Other Compatibilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Bead size ~100 μm
Storage temperature 2-8 °C
Bead Ligand Co-NTA
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 6% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
Required Buffers for purification under native conditions
 
  • Native Lysis buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 10 mM, pH 8 Optional: Add 1 tablet protease inhibitor cocktail to the Lysis Buffer. Tip: Lysis Buffer contains 10 mM imidazole to prevent binding of untagged proteins. If His-tagged proteins do not bind under these conditions, reduce the imidazole concentration to 1-5 mM.
  • Native Wash buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 20 mM, pH 8
  • Native Elution buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 250- 500 mM, pH 8
Required Buffers for purification under denaturing conditions
 
  • Denaturing Lysis buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 8.0, Optional: Benzonase® nuclease (e.g. Merck Milipore, #707464)
  • Denaturing Wash buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 6.3NaH2PO4 100 mM
  • Denaturing Elution buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 4.5 Tip: If the target protein is acid-labile, elution can be performed with 250-500 mM imidazole. Note: Due to urea dissociation, adjust the pH immediately before use.
Top seller
Co-NTA Cartridges 100
No results were found for the filter!
PureCube 100 Co-NTA Cartridge PureCube 100 Co-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose with 100 micron average bead size, loaded with cobalt (II) chloride
Article number: 74601
Sales price: From €49.00 *
PureCube 100 Compact Cartridge Co-NTA PureCube 100 Compact Cartridge Co-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose with 100 micron average bead size, loaded with cobalt (II) chloride
Article number: 74602
Sales price: From €49.00 *
Viewed