Ni-IDA Cartridges


Agarose guide
The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech offers high-performance PureCube Ni-IDA , prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC). Do you prefer NTA? We offer the same high-quality resin with that chelating ligand! (what's the difference?)

PureCube Ni-IDA Cartridges provide:


Features

Feature Ni-NTA Cartridge Ni-NTA COMPACT Cartridge
Usage Purification of his tagged proteins
Specifity Affinity to His-tagged proteins
Technology IMAC
Functional Group Iminodiacetic Acid Iminodiacetic Acid
Protein Binding capacity
50 mg (1ml cartridge)
250 mg (5ml cartridge)
pH stability 2-14
Other Compatibilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Bead size 40 μm
Storage temperature 2-8 °C
Bead Ligand Ni-IDA
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 7.5% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
Required Buffers for purification under native conditions
 
  • Native Lysis buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 10 mM, pH 8 Optional: Add 1 tablet protease inhibitor cocktail to the Lysis Buffer. Tip: Lysis Buffer contains 10 mM imidazole to prevent binding of untagged proteins. If His-tagged proteins do not bind under these conditions, reduce the imidazole concentration to 1-5 mM.
  • Native Wash buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 20 mM, pH 8
  • Native Elution buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 250- 500 mM, pH 8
Required Buffers for purification under denaturing conditions
 
  • Denaturing Lysis buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 8.0, Optional: Benzonase® nuclease (e.g. Merck Milipore, #707464)
  • Denaturing Wash buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 6.3NaH2PO4 100 mM
  • Denaturing Elution buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 4.5 Tip: If the target protein is acid-labile, elution can be performed with 250-500 mM imidazole. Note: Due to urea dissociation, adjust the pH immediately before use.
Agarose guide
The His tag is the most widely used affinity tag due to its small size, low immunogenicity, and versatility under native or denaturing conditions, as well as in presence of detergents and many other additives. Cube Biotech offers high-performance PureCube Ni-IDA , prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC). Do you prefer NTA? We offer the same high-quality resin with that chelating ligand! (what's the difference?)

PureCube Ni-IDA Cartridges provide:


Features

Feature Ni-NTA Cartridge Ni-NTA COMPACT Cartridge
Usage Purification of his tagged proteins
Specifity Affinity to His-tagged proteins
Technology IMAC
Functional Group Iminodiacetic Acid Iminodiacetic Acid
Protein Binding capacity
50 mg (1ml cartridge)
250 mg (5ml cartridge)
pH stability 2-14
Other Compatibilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile
Bead size 40 μm
Storage temperature 2-8 °C
Bead Ligand Ni-IDA
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 7.5% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
Required Buffers for purification under native conditions
 
  • Native Lysis buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 10 mM, pH 8 Optional: Add 1 tablet protease inhibitor cocktail to the Lysis Buffer. Tip: Lysis Buffer contains 10 mM imidazole to prevent binding of untagged proteins. If His-tagged proteins do not bind under these conditions, reduce the imidazole concentration to 1-5 mM.
  • Native Wash buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 20 mM, pH 8
  • Native Elution buffer: NaH2PO4 50 mM, NaCl 300 mM, Imidazole 250- 500 mM, pH 8
Required Buffers for purification under denaturing conditions
 
  • Denaturing Lysis buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 8.0, Optional: Benzonase® nuclease (e.g. Merck Milipore, #707464)
  • Denaturing Wash buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 6.3NaH2PO4 100 mM
  • Denaturing Elution buffer: NaH2PO4 100 mM, Tris base 10 mM, Urea 8 M, pH 4.5 Tip: If the target protein is acid-labile, elution can be performed with 250-500 mM imidazole. Note: Due to urea dissociation, adjust the pH immediately before use.
Top seller
Ni-IDA Cartridges
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PureCube Ni-IDA Cartridge PureCube Ni-IDA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30301
Sales price: From €32.00 *
PureCube Compact Cartridge Ni-IDA PureCube Compact Cartridge Ni-IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30302
Sales price: From €32.00 *
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