StrepTactin® Columns for purification | Cube Biotech

Strep Affinity Cartridges


FPLC Columns

PureCube HiCap StrepTactin® Cartridges and Compact Cartridges

The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. Cube Biotech offers a high-quality affinity resin for strep-tagged proteins, with maximum binding capacity, that is prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC). For detection of strep-tagged proteins in Western Blots specific antibodies and antibody conjugates are available.

The HiCap StrepTactin affinity catridges provide:

  • Binding capacity of up to 5 mg protein per mL resin
  • Stable in the presence of a wide range of additives
  • Two cartridge geometries: Standard and Compact
  • Compatible with common chromatography instruments
  • Excellent flow distribution and minimal void volume

Features

Feature PureCube Strep® affinity Cartridge, 1 & 5 mL Purecube Strep® affinity COMPACT Cartridge, 1& 5 mL
Usage Purification of Strep&reg tagged proteins
Specifity Affinity to Strep&reg tagged proteins
Technology IMAC
Protein Binding capacity
5 mg (1ml cartridge)
25 mg (5ml cartridge)
pH stability 3-12
Other Compatibilities 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, 2% n-Octyl-b-D-glucopyranoside, 0.2% n-Nonyl-b-D-glucopyranoside, 0.35% n-Decyl-b-D-maltoside, 2% Lauryl-sarcosine, 0.1% SDS, 0.3% CHAPS, 1M Guanidine Hcl, 1mM PMSF, 10% ethanol, 5M NaCl, 2M (NH4)2SO4, 1M CaCl2, 25% glycerol
Bead size ~50-150 μm
Storage temperature 2-8 °C
Functional Group Streptactin®
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 6% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
FPLC Columns

PureCube HiCap StrepTactin® Cartridges and Compact Cartridges

The Strep-tag® purification system is based on the highly selective binding of engineered streptavidin, called Strep-Tactin, to Strep-tag II fusion proteins. Cube Biotech offers a high-quality affinity resin for strep-tagged proteins, with maximum binding capacity, that is prepacked in affinity chromatography columns with 1 and 5 mL volume, ready to be used on automated liquid chromatography systems (e.g., FPLC). For detection of strep-tagged proteins in Western Blots specific antibodies and antibody conjugates are available.

The HiCap StrepTactin affinity catridges provide:

  • Binding capacity of up to 5 mg protein per mL resin
  • Stable in the presence of a wide range of additives
  • Two cartridge geometries: Standard and Compact
  • Compatible with common chromatography instruments
  • Excellent flow distribution and minimal void volume

Features

Feature PureCube Strep® affinity Cartridge, 1 & 5 mL Purecube Strep® affinity COMPACT Cartridge, 1& 5 mL
Usage Purification of Strep&reg tagged proteins
Specifity Affinity to Strep&reg tagged proteins
Technology IMAC
Protein Binding capacity
5 mg (1ml cartridge)
25 mg (5ml cartridge)
pH stability 3-12
Other Compatibilities 2% Tween 20, 2% Triton X-100, 2% IGEPAL® 630/Nonidet P40, 2% n-Octyl-b-D-glucopyranoside, 0.2% n-Nonyl-b-D-glucopyranoside, 0.35% n-Decyl-b-D-maltoside, 2% Lauryl-sarcosine, 0.1% SDS, 0.3% CHAPS, 1M Guanidine Hcl, 1mM PMSF, 10% ethanol, 5M NaCl, 2M (NH4)2SO4, 1M CaCl2, 25% glycerol
Bead size ~50-150 μm
Storage temperature 2-8 °C
Functional Group Streptactin®
Bed Volume 1 ml or 5 ml
Dimensions of cartridges
6.2mm X 50 mm (1ml cartridge)
11mm X 80 mm (5ml Cartridge)
5mm X 35 mm (1ml cartridge)
17mm X 35mm (5ml cartridge)
Column Body Material
Polypropylene (1ml cartridge)
Acrylate (5ml cartridge)
Polypropylene
Inlet 10-32 UNF female thread
Outlet 10-32 UNF female thread
Matrix 6% highly cross-linked agarose
Max. Flow Rate 6 mL/min
Recommended Flow Rate 0.5-2.0 mL/min
Recommended Operational Pressure
Up to 5 bar (72 psi) (1ml cartridge)
Up to 3 bar (42 psi) (5ml cartridge)
Strep Affinity Cartridges
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PureCube HiCap StrepTactin Cartridge PureCube HiCap StrepTactin Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml high capacity Streptactin agarose  
Article number: 34301
Sales price: From €106.00 *
PureCube Compact Cartridge HiCap StrepTactin PureCube Compact Cartridge HiCap StrepTactin
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml high capacity Streptactin agarose
Article number: 34302
Sales price: From €106.00 *
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