Protein Purification Products


Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His tagged proteins
Glutathione / GST tag Agarose & MagBeads The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific./td> Strep tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! rho1d4 tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS Activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine Activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy Activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide Activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
Top seller
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Article number: 74103
PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
Article number: 75201
Rho1D4 peptide Rho1D4 peptide
Article number: 16201
PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
Article number: 31201
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
Article number: 40020
Protein Purification Products
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Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
For the detection of Rho1D4 tagged proteins on westernblots. Our Rho1D4 affinity antibody, from mouse!
Article number: 40020
Sales price: €279.00 *
Strep antibody (0.1 mg) Strep antibody (0.1 mg)
For the detection of Strep tagged proteins on westernblots. Our Strep affinity antibody, from mouse!
Article number: 40070
Sales price: €196.00 *
PentaHis antibody, BSA-free (0.1 mg) PentaHis antibody, BSA-free (0.1 mg)
Lyophilized. Manufactured by QIAGEN.  
Article number: 40040
Sales price: €448.00 *
GST antibody (0.1 mg) GST antibody (0.1 mg)
GST antibody, lyophilized.For immunoblotting and westernblotting of GST tagged proteins. From Mouse.  
Article number: 40060
Sales price: €476.00 *
Strep antibody HRP Strep antibody HRP
For the detection of Strep tagged proteins on westernblots & more. Our Strep HRP affinity antibody, from mouse!
Article number: 40071
Sales price: €248.00 *
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