Protein Purification Products


Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His tagged proteins
Glutathione / GST tag Agarose & MagBeads The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific./td> Strep tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! rho1d4 tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS Activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine Activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy Activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide Activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
Top seller
PureCube Ni-NTA Agarose PureCube Ni-NTA Agarose
Article number: 31103
PureCube 100 Ni-NTA Agarose PureCube 100 Ni-NTA Agarose
Article number: 74103
PureCube INDIGO Ni-MagBeads PureCube INDIGO Ni-MagBeads
Article number: 75201
Rho1D4 peptide Rho1D4 peptide
Article number: 16201
PureCube Ni-NTA Mag Beads PureCube Ni-NTA Mag Beads
Article number: 31201
Rho1D4 antibody (0.2 mg) Rho1D4 antibody (0.2 mg)
Article number: 40020
Protein Purification Products
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PureCube Cu-NTA Cartridge PureCube Cu-NTA Cartridge
Pre-Filled FPLC column with Copper-NTA Agarose. Perfect for high-yield His Tag purification and copper binding enrichment.
Article number: 31601-Cu
Sales price: From €37.00 *
PureCube Fe-NTA Cartridge PureCube Fe-NTA Cartridge
PureCube High quality Fe-NTA agarose resin pre-packed in columns for the enrichment of phosphorylated peptides.
Article number: 31601-Fe
Sales price: From €37.00 *
PureCube Zn-NTA Cartridge PureCube Zn-NTA Cartridge
Zn-NTA agarose resin beads pre-filled in FPLC columns / cartridges for the enrichment of zinc-ion binding proteins via FPLC.
Article number: 31601-Zn
Sales price: From €37.00 *
PureCube Compact Cartridge Al-NTA PureCube Compact Cartridge Al-NTA
Column for low pressure chromatography (FPLC), pre-filled with Al-NTA agarose resin beads for phosphopeptide enrichment.
Article number: 31602-Al
Sales price: From €37.00 *
PureCube Compact Cartridge Fe-NTA PureCube Compact Cartridge Fe-NTA
PureCube High quality Fe-NTA agarose resin pre-packed in COMPACT cartridges for the enrichment of phosphorylated peptides.
Article number: 31602-Fe
Sales price: From €37.00 *
PureCube Compact Cartridge Zn-NTA PureCube Compact Cartridge Zn-NTA
Zn-NTA agarose resin beads pre-filled in FPLC compact cartridges for the enrichment of zinc-ion binding proteins via FPLC.
Article number: 31602-Zn
Sales price: From €37.00 *
PureCube Glutathione Cartridge PureCube Glutathione Cartridge
Cube Biotech offers GST Tag affinity columns for FPLC based affinity protein purification. Pre-packed cartridges for your use
Article number: 32301
Sales price: From €40.00 *
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