Protein Purification Products

Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.

Table 1: Different affinities for different applications

Affinity	Specification	Protein binding
His-tag-Affinity Agarose & MagBeads	Due to its very small size it rarely affects protein function. High protein yields!	His tagged proteins
Glutathione / GST tag Agarose & MagBeads	The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding.	GST tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads	The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific./td>	Strep tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads	The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag!	rho1d4 tagged proteins
Hydrophobic Interaction Agarose	Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein.	Hydrophobic amino acid residues.
Alprenolol Resins	A resin for a highly spezialized purpose.	ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.

Table 2: Special IMAC resins / MagBeads for more specialized purposes.

Products	Description	Affinity
Cu-NTA Agarose / MagBeads	Cu²⁺ coupled to NTA ligand	Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads	Zn²⁺ coupled to NTA ligand	Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.

Table 3: Activated resins and MagBeads for specialized applications.

Active group	Specification	Coupling to which group
Carboxy Agarose & MagBeads	Resin carrys a free carboxyl group	Amine
NHS Activated Agarose & MagBeads	Resin carrys a N-hydroxy-succinimide (NHS) functionality	Amine
Amine Activated Agarose & MagBeads	Resin carrys a free amino group	Carboxy
Epoxy Activated Agarose & MagBeads	Resin carrys a free epoxy group	Amine or Thiol
Maleimide Activated Agarose & MagBeads	Resin carrys a free maleimide group	Thiol