Protein Purification Products


Once expressed proteins are extracted from cell lysates or membranes, affinity chromatography is an elegant way to purify these to homogeneity in very few steps. There are multiple ways to gain this homogeneity, but mostly affinity tags are used in together with a matching purification resin. Cube Biotech offers a broad range of purification agarose resins or magnetic beads for the purification of functional protein. See below what types of resin and magnetic beads we offer.

Not sure which tag to use? See our overview table HERE.

Additionally we offer activated resins and MagBeads for coupling to amino, thiol and carboxy groups directly.
 

Table 1: Different affinities for different applications
AffinitySpecificationProtein binding
His-tag-Affinity Agarose & MagBeads Due to its very small size it rarely affects protein function. High protein yields! His tagged proteins
Glutathione / GST tag Agarose & MagBeads The GST tag is a 26 kDa protein tag which is often chosen to promote recombinant protein folding. GST tagged proteins (Glutathione-S-Tranferase)
Strep-tag Affinity Agarose & MagBeads The Strep-tag® is a 8 amino acid tag with high specificity for StrepTactin®. Highly specific./td> Strep tagged proteins
Rho1D4 / 1D4 tag Affinity Agarose & MagBeads The rho1D4-tag is a 9 amino acid tag that binds to the rho1D4 antibody. Our most specific affinity tag! rho1d4 tagged proteins
Hydrophobic Interaction Agarose Agarose resin / MagBeads that carry a hydrophobic group for matching interactions with a protein. Hydrophobic amino acid residues.
Alprenolol Resins A resin for a highly spezialized purpose. ß-adrenergic receptors and alkene functions

Furthermore we offer more specialized IMAC resins and other affinities coupled to the NTA or IDA ligand.
 
Table 2: Special IMAC resins / MagBeads for more specialized purposes.
ProductsDescriptionAffinity
Cu-NTA Agarose / MagBeads Cu2+ coupled to NTA ligand Copper-binding and His-tagged proteins
Zn-NTA Agarose / MagBeads Zn2+ coupled to NTA ligand Zinc-binding (e.g Zinc-finger) proteins

Our Activated resins offer a wide selection of different active groups on the resin / MagBead. A possible application these products is the coupling of an antibody to them.
Table 3: Activated resins and MagBeads for specialized applications.
Active groupSpecificationCoupling to which group
Carboxy Agarose & MagBeads Resin carrys a free carboxyl group Amine
NHS Activated Agarose & MagBeads Resin carrys a N-hydroxy-succinimide (NHS) functionality Amine
Amine Activated Agarose & MagBeads Resin carrys a free amino group Carboxy
Epoxy Activated Agarose & MagBeads Resin carrys a free epoxy group Amine or Thiol
Maleimide Activated Agarose & MagBeads Resin carrys a free maleimide group Thiol
 
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Protein Purification Products
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PureCube Ni-IDA MagBeads PureCube Ni-IDA MagBeads
1 ml 25% IDA magnetic beads, loaded with nickel (II) sulfate
Article number: 30201
Sales price: From €43.00 *
PureCube Ni-IDA Cartridge PureCube Ni-IDA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30301
Sales price: From €33.00 *
PureCube Compact Cartridge Ni-IDA PureCube Compact Cartridge Ni-IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, loaded with nickel (II) sulfate
Article number: 30302
Sales price: From €33.00 *
PureCube IDA Agarose PureCube IDA Agarose
2 ml 50% IDA Agarose - for loading with transition metals
Article number: 30703
Sales price: From €70.00 *
PureCube IDA MagBeads PureCube IDA MagBeads
1 ml 25% IDA Magnetic Beads - for loading with transition metals
Article number: 30801
Sales price: From €43.00 *
PureCube IDA Cartridge PureCube IDA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, for loading with transition metals
Article number: 30901
Sales price: From €33.00 *
PureCube Compact Cartridge IDA PureCube Compact Cartridge IDA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml IDA Agarose, for loading with transition metals
Article number: 30904
Sales price: From €33.00 *
PureCube Ni-NTA Cartridge PureCube Ni-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with nickel (II) sulfate
Article number: 31301
Sales price: From €34.00 *
PureCube Co-NTA Cartridge PureCube Co-NTA Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with cobalt (II) chloride
Article number: 31601
Sales price: From €37.00 *
PureCube Compact Cartridge Co-NTA PureCube Compact Cartridge Co-NTA
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml NTA Agarose, loaded with cobalt (II) chloride
Article number: 31602
Sales price: From €37.00 *
PureCube NTA Agarose PureCube NTA Agarose
2 ml 50% NTA Agarose - for loading with transition metals
Article number: 31701
Sales price: From €13.00 *
PureCube NTA MagBeads PureCube NTA MagBeads
1 ml 25% NTA Magnetic Beads - for loading with transition metals
Article number: 31801
Sales price: From €63.00 *
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