PureCube Compact Cartridge IDA

Order number: 30906

€164.00*

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Description

PureCube IDA (iminodiacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 40 µm and thus great mechanical stability.
Since the affinity of an IDA-metal-ion-complex depends on the loaded metal ion, we offer IDA agarose resin uncoupled to any metal ion for the case that we do not offer your desired IDA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.

PureCube IDA resin is also available with a bead diameter of 400 µm for higher FPLC flow rates or asIDA magnetic beads. Furthermore, PureCube IDA agarose can also be obtained pre-packed into FPLC cartridges/columns.
The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA TM.

However, we already have a vast collection of IDA and NTA-based beads in our store. Maybe we already have what you need.
Feature IDA Resin
Usage For coupling of metal ions for corresponding purification and / or enrichment purposes
Specificity Varies
Binding capacity Depends on the loaded ion
Purity Depends on the loaded ion
Bead Ligand IDA
Bead size 40 μm
Matrix 7.5% cross-linked agarose
Filling quantity Delivered as a 50 % suspension
Recommended FPLC flow rates 0.5-2 ml/min
Maximal FPLC flow rate 6 ml/min
pH stability 2-14
Other stabilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile


Feature - Column
PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Max. Flow Rate 6 mL/min 6 mL/min
Dimensions: diameter X length (mm) 5 X 35 17 X 35
Body material Polypropylene Polypropylene
Inlet 10-32 UNF female thread 10-32 UNF female thread
Outlet 10-32 UNF female thread 10-32 UNF female thread

FAQ

Can I get the datasheet for the PureCube IDA resin?

Why is there a special protocol for the loading of nickel ions?

As Ni-IDA is the most commonly used IDA product, we have optimized this specific protocol.

Can I order a specific IDA product from you, even if it is not in your shop?

Yes, we offer a custom bead service for that.