PureCube NTA Agarose

Order number: 31705

€535.00*

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Description

PureCube NTA (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 40 µm and thus great mechanical stability.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.

PureCube NTA resin is also available with a bead diameter of 100 µm or even XL sized (400 µm) for higher FPLC flow rates or as NTA magnetic beads. Furthermore, PureCube NTA agarose can also be obtained pre-packed into FPLC cartridges/columns.

However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
Feature
Usage For coupling of metal ions for corresponding purification and / or enrichment purposes
Specificity Varies
Binding capacity Depends on the loaded ion
Purity Depends on the loaded ion
Bead Ligand NTA
Bead size 40 μm
Matrix 7.5% cross-linked agarose
Filling quantity Delivered as a 50 % suspension
Recommended FPLC flow rates 0.5-2 ml/min
Maximal FPLC flow rate 6 ml/min
pH stability 2-14
Other stabilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

Citations

Coupled molecule / metal ionPurified SubstanceYearAuthor
Gallium Phages 2019 Schönberger N., Braun R., Matys S., Lederer F-L., Lehmann F., Flemming K., Pollmann K.
Gallium Gallium binding Peptides 2021 Schönberger N.

FAQ

Can I get the datasheet for the PureCube NTA resin?

Why is there a special protocol for the loading of nickel ions?

As Ni-NTA is the most commonly used NTA product, we have optimized this specific protocol.

Can I order a specific NTA product from you, even if it is not in your shop?

Yes, we offer a custom bead service for that.