PureCube NTA Agarose XL

Order number: 55810

€2,905.00*

Ready to ship today
Quantity

Description

PureCube NTA XL (nitrilotriacetic acid) agarose resin was developed for IMAC-based protein purification and protein enrichment methods. The beads in this suspension have an average diameter of 400 µm. This is very large and is meant for very high flow rates in e.g. FPLC assays or very viscous cell media.
Since the affinity of an NTA-metal-ion-complex depends on the loaded metal ion, we offer NTA agarose resin uncoupled to any metal ion for the case that we do not offer your desired NTA + metal ion combination. Through our coupling protocol, you can in turn attach your desired metal ions to the agarose beads.

PureCube NTA resin is also available with a bead diameter of 40 µm or 100 µm for greater mechanical stability or as NTA magnetic beads.

However, we already have a vast collection of NTA-based beads in our store. Maybe we already have what you need.
Feature
Usage For coupling of metal ions for corresponding purification and / or enrichment purposes
Specificity Varies
Binding capacity Depends on the loaded ion
Purity Depends on the loaded ion
Bead Ligand NTA
Bead size 400 μm
Matrix 7.5% cross-linked agarose
Filling quantity Delivered as a 50 % suspension
Recommended FPLC flow rates 0.5-2 ml/min
Maximal FPLC flow rate 6 ml/min
pH stability 2-14
Other stabilities 100% methanol, 100% ethanol, 8 M urea, 6 M guanidinium hydrochloride, 30% (v/v) acetonitrile

FAQ

Can I get the datasheet for the PureCube NTA XL resin?

Why is there a special protocol for the loading of nickel ions?

As Ni-NTA is the most commonly used NTA product, we have optimized this specific protocol.

Can I order a specific NTA product from you, even if it is not in your shop?

Yes, we offer a custom bead service for that.