Glutathione


Purification Resins
The GST tag is a 26 kDA sized protein tag that can be used for protein purification. GST is short for Glutathione-S-Transferase. Using the GST tag for protein purification is taking advantage of an enzyme's specificity to a substrate to gain maximum purity.

Overview of Cube Biotech's Glutathione products for GST tag protein purification.


Features

Usage Specific binding and purification of GST-tagged proteins
Specifity Affinity to GST-tagged proteins
High binding capacity 10 mg / ml
Bead Ligand Protein purification beads with GST-Antibody

GST / Gluthathione-affinity agarose resins from Cube Biotech were successfully used in the following publications:

 ProteinYearAuthor
Human LGP2, RIG‐I, MDA5 and LGP2 CTD 2018   van der Veen A.G., Maillard P.V., Schmidt J.M., Lee S.A., Deddouche-Grass S., Borg A., Kjaer S., Snijders A.P., Reis e Sousa C.1
Human DNA polymerase α-primase and B- fusion proteins 2017  Kilkenny M.L., Simon A.C., Mainwaring J., Wirthensohn D., Holzer S., Pellegrini L.2
ISG15 2018  Napolitano A., van der Veen A.G., Bunyan M., Borg A., Frith D., Howell S., kjaer S., Beling A., Snijders A.P., Knobeloch K-P., Frickel E-M.3
Ran (wt) Ran KxQ/R; NTF2; RCC1 49-444; Importin- beta, Ran, KxAcK 2019  de Boor S.4
several TBX3 fusion proteins 2015  Fischer K.5
GtPEBB, mGtPEBB and variants 2019 Sommerkamp J.A.; Frankenberg-Dinkel N.; Hofmann E.6
Several in vitro synthesized proteins 2016 Chiocchini C., Trefzer A., Vattem K., Kuhn P., 7

PureCube Gluathione MagBead's efficiency

Gluthathione MagBeads efficiency
Fig.1: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.
Our GST tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST tag purification with the same beads from is ensured to be of the same high quality.

References:

  1. Van der Veen, A.G. et al. “The RIG-I-like receptor LGP2 inhibits Dicer-dependent processing of long double-stranded RNA and blocks RNA interference in mammalian cells.” The EMBO journal vol. 37,4 (2018): e97479. doi:10.15252/embj.201797479
  2. Kilkenny, M. L et al. “The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box.” Open biology vol. 7,11 (2017): 170217. doi:10.1098/rsob.170217.
  3. Napolitano, Anna et al. “Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection.” Journal of immunology (Baltimore, Md. : 1950) vol. 201,2 (2018): 604-614. doi:10.4049/jimmunol.1701322
  4. Boor, Susanne de (2015). Mechanistic studies on the regulation of Ran-function by post-translational lysine-acetylation. Dissertation, Universität zu Köln.
  5. Fischer K. (2015). TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Dissertation, Universität zu Mainz.
  6. A. Sommerkamp, et al. (2019). Crystal structure of the first eukaryotic bilin reductase Gt PEBB reveals a flipped binding mode of dihydrobiliverdin. Journal of Biological Chemistry. jbc.RA119.009306. 10.1074/jbc.RA119.009306.
  7. Chiocchini C., Trefzer A., Vattem K., Kuhn P., inventor; Thermo Fisher Scientific Geneart GmBH, Pierce Biotechnology Inc, assignee. Devices and methods for producing nucleic acids and proteins. United States patent US20180291413A1. 2016 April 10.
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Glutathione / GST tag
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PureCube Glutathione MagBeads PureCube Glutathione MagBeads
1 ml 25% Glutathione Magnetic Beads For more information read here: https://cube-biotech.com/products/magnetic-beads/gst-affinity-magbeads/#
Article number: 32205
Sales price: From €50.00 *
PureCube Glutathione Agarose PureCube Glutathione Agarose
2 ml 50% Glutathione Agarose
Article number: 32103
Sales price: From €140.00 *
PureCube Compact Cartridge Glutathione PureCube Compact Cartridge Glutathione
Compact Cartridge for low pressure chromatography (FPLC), packed with 1 ml Glutathione Agarose
Article number: 32302
Sales price: From €40.00 *
GST antibody (0.1 mg) GST antibody (0.1 mg)
GST antibody, lyophilized.For immunoblotting and westernblotting of GST tagged proteins. From Mouse.  
Article number: 40060
Sales price: €476.00 *
PureCube Glutathione Agarose XL PureCube Glutathione Agarose XL
2 ml 50% Glutathione Agarose with medium particle size of 400 micrometer, for special applications
Article number: 57003
Sales price: From €178.00 *
PureCube Glutathione MagBeads XL PureCube Glutathione MagBeads XL
1 ml 25% Glutathione magnetic beads. Particle size 70-120 micrometer, for special applications.
Article number: 57105
Sales price: From €238.00 *
L-Glutathione oxidized L-Glutathione oxidized
Ultrapure and ready to use ► To create redox potentials for chemical reactions.| Cystein source for the cells.
Article number: 61003
Sales price: From €111.00 *
L-Glutathione reduced L-Glutathione reduced
Reduced L-Glutathione ►Ultrapure and convenient for GST-tagged protein elution from Glutathione Agarose or MagBeads.
Article number: 61035
Sales price: €111.00 *
PureCube Glutathione Cartridge PureCube Glutathione Cartridge
Cartridge for low pressure chromatography (FPLC), packed with 1 ml Glutathione Agarose
Article number: 32301
Sales price: From €40.00 *
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