Glutathione

The GST tag is a 26 kDA sized protein tag that can be used for protein purification. GST is short for Glutathione-S-Transferase. Using the GST tag for protein purification is taking advantage of an enzyme's specificity to a substrate to gain maximum purity.

Overview of Cube Biotech's Glutathione products for GST tag protein purification.


	Glutathione protein purification products
Glutathione agarose resin (40 µm)	Glutathione agarose (40 µm) Cartridge	Glutathione MagBeads (30 µm)
Glutathione agarose resin (XL sized)	Glutathione agarose (40 µm) COMPACT Cartridge	Glutathione MagBeads (XL sized)
	Glutathione protein purification support products
L-Glutathione [reduced] (for protein elution)	GST and Rho1D4 tagged GFP(for positive controls)	GST antibody [mouse] (for blots)
L-Glutathione [oxidized]	GST and DYKDDDDK tagged GFP(for positive controls)

Features

Usage	Specific binding and purification of GST-tagged proteins
Specifity	Affinity to GST-tagged proteins
High binding capacity	10 mg / ml
Bead Ligand	Protein purification beads with GST-Antibody

GST / Gluthathione-affinity agarose resins from Cube Biotech were successfully used in the following publications:

Protein	Year	Author
Human LGP2, RIG‐I, MDA5 and LGP2 CTD	2018	van der Veen A.G., Maillard P.V., Schmidt J.M., Lee S.A., Deddouche-Grass S., Borg A., Kjaer S., Snijders A.P., Reis e Sousa C.¹
Human DNA polymerase α-primase and B- fusion proteins	2017	Kilkenny M.L., Simon A.C., Mainwaring J., Wirthensohn D., Holzer S., Pellegrini L.²
ISG15	2018	Napolitano A., van der Veen A.G., Bunyan M., Borg A., Frith D., Howell S., kjaer S., Beling A., Snijders A.P., Knobeloch K-P., Frickel E-M.³
Ran (wt) Ran KxQ/R; NTF2; RCC1 49-444; Importin-beta, Ran, KxAcK	2019	de Boor S.⁴
several TBX3 fusion proteins	2015	Fischer K.⁵
GtPEBB, mGtPEBB and variants	2019	Sommerkamp J.A.; Frankenberg-Dinkel N.; Hofmann E.⁶
Several in vitro synthesized proteins	2016	Chiocchini C., Trefzer A., Vattem K., Kuhn P., ⁷

PureCube Gluathione MagBead's efficiency

Fig.1: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.

Our GST tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST tag purification with the same beads from is ensured to be of the same high quality.

References:

Van der Veen, A.G. et al. “The RIG-I-like receptor LGP2 inhibits Dicer-dependent processing of long double-stranded RNA and blocks RNA interference in mammalian cells.” The EMBO journal vol. 37,4 (2018): e97479. doi:10.15252/embj.201797479
Kilkenny, M. L et al. “The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box.” Open biology vol. 7,11 (2017): 170217. doi:10.1098/rsob.170217.
Napolitano, Anna et al. “Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection.” Journal of immunology (Baltimore, Md. : 1950) vol. 201,2 (2018): 604-614. doi:10.4049/jimmunol.1701322
Boor, Susanne de (2015). Mechanistic studies on the regulation of Ran-function by post-translational lysine-acetylation. Dissertation, Universität zu Köln.
Fischer K. (2015). TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Dissertation, Universität zu Mainz.
A. Sommerkamp, et al. (2019). Crystal structure of the first eukaryotic bilin reductase Gt PEBB reveals a flipped binding mode of dihydrobiliverdin. Journal of Biological Chemistry. jbc.RA119.009306. 10.1074/jbc.RA119.009306.
Chiocchini C., Trefzer A., Vattem K., Kuhn P., inventor; Thermo Fisher Scientific Geneart GmBH, Pierce Biotechnology Inc, assignee. Devices and methods for producing nucleic acids and proteins. United States patent US20180291413A1. 2016 April 10.

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