Glutathione


Purification Resins
The GST tag is a 26 kDA sized protein tag that can be used for protein purification. GST is short for Glutathione-S-Transferase. Using the GST tag for protein purification is taking advantage of an enzyme's specificity to a substrate to gain maximum purity. If you want to learn more about the GST tag read our comprehensive guide.

Overview of Cube Biotech's Glutathione products for GST tag protein purification.


Features

Usage Specific binding and purification of GST-tagged proteins
Specifity Affinity to GST-tagged proteins
High binding capacity 10 mg / ml
Bead Ligand Protein purification beads with GST-Antibody

GST / Gluthathione-affinity agarose resins from Cube Biotech were successfully used in the following publications:

 ProteinYearAuthorUsed Product
Human DNA polymerase α-primase and B- fusion proteins 2017  Kilkenny M.L., Simon A.C., Mainwaring J., Wirthensohn D., Holzer S., Pellegrini L.1 Gluthathione Agarose
ISG15 2018  Napolitano A., van der Veen A.G., Bunyan M., Borg A., Frith D., Howell S., kjaer S., Beling A., Snijders A.P., Knobeloch K-P., Frickel E-M.2 Gluthathione Agarose
Ran (wt) Ran KxQ/R; NTF2; RCC1 49-444; Importin- beta, Ran, KxAcK 2019  de Boor S.3 Gluthathione Agarose
several TBX3 fusion proteins 2015  Fischer K.4 Gluthathione Agarose & L-Glutathione (reduced)
GtPEBB, mGtPEBB and variants 2019 Sommerkamp J.A.; Frankenberg-Dinkel N.; Hofmann E.5 Gluthathione Agarose
Several in vitro synthesized proteins 2016 Chiocchini C., Trefzer A., Vattem K., Kuhn P., 6 Gluthathione MagBeads
Several GST tagged proteins 2020 Chiocchini C., Vattem K., Liss M., Ludewig L., Reusch T., Rastogi I., Webb B., Trefzer A., 7 Gluthathione MagBeads

PureCube Gluathione MagBead's efficiency

Gluthathione MagBeads efficiency
Fig.1: Reliable lot-to-lot reproducibility. Glutathione-S-transferase was purified from E.coli lysates using three independent production lots of PureCube Glutathione MagBeads. FT: Flow through, E1-E5: Elution fractions.
Our GST tag purification products are able to reproduce all purification assays from lot to lot. We achieved minimal fluctuations between multiple uses of our beads. Multiple consecutive GST tag purification with the same beads from is ensured to be of the same high quality.

References:

  1. Kilkenny, M. L et al. “The human CTF4-orthologue AND-1 interacts with DNA polymerase α/primase via its unique C-terminal HMG box.” Open biology vol. 7,11 (2017): 170217. doi:10.1098/rsob.170217.
  2. Napolitano, Anna et al. “Cysteine-Reactive Free ISG15 Generates IL-1β-Producing CD8α+ Dendritic Cells at the Site of Infection.” Journal of immunology (Baltimore, Md. : 1950) vol. 201,2 (2018): 604-614. doi:10.4049/jimmunol.1701322
  3. Boor, Susanne de (2015). Mechanistic studies on the regulation of Ran-function by post-translational lysine-acetylation. Dissertation, Universität zu Köln.
  4. Fischer K. (2015). TBX3-Mutationsanalysen und Konstruktion einer Zelllinie für TBX2-Inhibitorscreenings. Dissertation, Universität zu Mainz.
  5. A. Sommerkamp, et al. (2019). Crystal structure of the first eukaryotic bilin reductase Gt PEBB reveals a flipped binding mode of dihydrobiliverdin. Journal of Biological Chemistry. jbc.RA119.009306. 10.1074/jbc.RA119.009306.
  6. Chiocchini C., Trefzer A., Vattem K., Kuhn P., inventor; Thermo Fisher Scientific Geneart GmBH, Pierce Biotechnology Inc, assignee. Devices and methods for producing nucleic acids and proteins. United States patent US20180291413A1. 2016 April 10.
  7. Chiocchini, C. et al. (2020). From Electronic Sequence to Purified Protein Using Automated Gene Synthesis and In Vitro Transcription/Translation. ACS Synthetic Biology. XXXX. 10.1021/acssynbio.0c00060.
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Glutathione / GST tag
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PureCube Glutathione Agarose PureCube Glutathione Agarose
Cube Biotech offers PureCube GST Agarose resin for the protein Purification of GST tagged proteins | 10 mg protein / ml resin
Article number: 32103
Sales price: From €112.00 * €140.00 *
PureCube Glutathione MagBeads PureCube Glutathione MagBeads
Cube Biotech offers PureCube GST MagBeads for the protein Purification of GST tagged proteins | 10 mg protein / ml MagBeads
Article number: 32205
Sales price: From €50.00 *
PureCube Glutathione Cartridge PureCube Glutathione Cartridge
Cube Biotech offers GST Tag affinity columns for FPLC based affinity protein purification. Pre-packed cartridges for your use
Article number: 32301
Sales price: From €40.00 *
L-Glutathione reduced L-Glutathione reduced
Reduced L-Glutathione ►Ultrapure and convenient for GST-tagged protein elution from Glutathione Agarose or MagBeads.
Article number: 61035
Sales price: €111.00 *
PureCube Compact Cartridge Glutathione PureCube Compact Cartridge Glutathione
Cube Biotech offers GST Tag affinity columns for FPLC based affinity protein purification. Pre-packed COMPACT cartridges.
Article number: 32302
Sales price: From €40.00 *
GST antibody (0.1 mg) GST antibody (0.1 mg)
GST antibody, lyophilized.For immunoblotting and westernblotting of GST tagged proteins. From Mouse.  
Article number: 40060
Sales price: €476.00 *
PureCube Glutathione Agarose XL PureCube Glutathione Agarose XL
Cube Biotech's PureCube GST Agarose XL resin for the protein Purification of GST tagged proteins | 2 mg protein / ml resin
Article number: 57003
Sales price: From €178.00 *
PureCube Glutathione MagBeads XL PureCube Glutathione MagBeads XL
Cube Biotech's PureCube GST MagBeads XL resin for the protein Purification of GST tagged proteins | 2 mg protein / ml beads
Article number: 57105
Sales price: From €238.00 *
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