PureCube Glutathione Agarose XL

PureCube Glutathione Agarose XL
€207.00 *

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mL:

  • 57003
PureCube Glutathione XL MagBeads were created with the purpose to increase FPLC flow rates... more
Product information "PureCube Glutathione Agarose XL"
Type: Agarose
Bead size: 400 µm (XL Agarose)
Affinity : GST-tag
Purpose: Protein Purification
PureCube Glutathione XL MagBeads were created with the purpose to increase FPLC flow rates and the performance of the resin beads in more viscous cell lysates. The beads have an average diameter of 400 µm and provide a protein yield of 2 mg / ml resin. If the advantages of XL resin beads are not important we recommend our regular sized PureCube Glutathione Agarose resin (Ø 40 µm).
As alternatives to agarose resins CubeBiotech also offers Gluthathione Magnetic Beads. These are also availible as a XL version.

Features

Usage Specific binding and purification of GST-tagged proteins
Specifity Affinity to GST (Gluthathione-S-Transferase)-tagged proteins
Binding capacity >2  mg/mL
Bead Ligand L-Glutathione (reduced)
Bead size Ø 400 µm
Stabilities 1 M sodium acetate, pH 4.0, or 6 M guanidinehydrochloride, organic solvents (e.g., 70% (v/v) ethanol), 1% (w/v) SDS, 0.1 M NaOH, 0.1 M HCl.
pH Stability 3-12
Filling quantity Delivered as a 50 % suspension
Material 6 % cross-linked agarose
Required equipment
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  • pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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