PureCube Co-NTA Agarose

PureCube Co-NTA Agarose
€88.80 * €111.00 * (20% Saved)

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mL:

  • 31403
Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm.... more
Product information "PureCube Co-NTA Agarose"
Type: Agarose
Bead size: 40 µm (Agarose)
Affinity : His-tag
Purpose: Protein Purification
Ligand: NTA
Coupled Ion: Co2+
Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm. Cobalt Ions that are coupled to NTA on the surface on the beads for purification of active His-tagged protein. Our agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent. For Co-NTA agarose resin products that enable higher flow rates we recommend our PureCube 100 Co-NTA resin and for extreme cases our Purecube XL Co-NTA agarose beads.

Features

Usage Specific binding and purification of 6x His-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >30 mg/mL
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Bead size 40 μm
Bead Ligand Co-NTA
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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