PureCube Co-NTA Agarose

PureCube Co-NTA Agarose
€107.00 *

Prices plus VAT plus shipping costs

Ready to ship today,
Delivery time appr. 1-3 workdays

ML:

  • 31403
Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm.... more
Product information "PureCube Co-NTA Agarose"
Type: Agarose
Bead size: 40 µm
Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm. Cobalt Ions that are coupled to NTA on the surface on the beads for purification of active His tagged protein. Our agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent. For Co-NTA agarose resin products that enable higher flow rates we recommend our PureCube 100 Co-NTA resin and for extreme cases our Purecube XL Co-NTA agarose beads.

Features

Usage Specific binding and purification of 6x his-tagged proteins
Specifity Affinity to His-tagged proteins
Binding capacity >30 mg/mL
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Bead size 40 μm
Bead Ligand Co-NTA
Required equipment
 
  • Lysis Buffer
  • Wash Buffer
  • Elution Buffer
  • Ice bath
  • Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
  • 50 mL centrifuge tube
  • Micropipettor and Micropipetting tips
  • Disposable gravity flow columns with capped bottom outlet, 2 ml
  •  pH meter
  • End-over-end shaker
  • SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
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