Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm. Cobalt Ions that are coupled to NTA on the surface on the beads for purification of active His tagged protein. Our agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent. For Co-NTA agarose resin products that enable higher flow rates we recommend our PureCube 100 Co-NTA resin and for extreme cases our Purecube XL Co-NTA agarose beads.
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Features
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity
Delivered as a 50 % suspension
Bead size
40 μm
Bead Ligand
Co-NTA
Required equipment
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
50 mL centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
