Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm. Cobalt Ions that are coupled to NTA on the surface on the beads for purification of active His-tagged protein. Our agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent. For Co-NTA agarose resin products that enable higher flow rates we recommend our PureCube 100 Co-NTA resin and for extreme cases our Purecube XL Co-NTA agarose beads.
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Features
Usage
Specific binding and purification of 6x His-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity
Delivered as a 50 % suspension
Bead size
40 μm
Bead Ligand
Co-NTA
Required equipment
Lysis Buffer
Wash Buffer
Elution Buffer
Ice bath
Refrigerated centrifuge for 50 mL tube (min 10,000 x g)
50 mL centrifuge tube
Micropipettor and Micropipetting tips
Disposable gravity flow columns with capped bottom outlet, 2 ml
pH meter
End-over-end shaker
SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment
