PureCube Co-NTA Agarose

€88.80 * €111.00 * (20% Saved)

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Order number: 31403

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Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm.... more

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Product information "PureCube Co-NTA Agarose"

Type:	Agarose
Bead size:	40 µm (Agarose)
Affinity :	His-tag
Purpose:	Protein Purification
Ligand:	NTA
Coupled Ion:	Co2+

Our PureCube Co-NTA agarose consist of small agarose resin beads with a diameter of 40 µm. Cobalt Ions that are coupled to NTA on the surface on the beads for purification of active His-tagged protein. Our agarose resins are suited for batch spin columns and FPLC. Due to their small size they are mechanically stable to a great extent. For Co-NTA agarose resin products that enable higher flow rates we recommend our PureCube 100 Co-NTA resin and for extreme cases our Purecube XL Co-NTA agarose beads.

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Features

Usage	Specific binding and purification of 6x His-tagged proteins
Specifity	Affinity to His-tagged proteins
Binding capacity	>30 mg/mL
Chelator stability	Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity	Delivered as a 50 % suspension
Bead size	40 μm
Bead Ligand	Co-NTA
Required equipment	Lysis Buffer Wash Buffer Elution Buffer Ice bath Refrigerated centrifuge for 50 mL tube (min 10,000 x g) 50 mL centrifuge tube Micropipettor and Micropipetting tips Disposable gravity flow columns with capped bottom outlet, 2 ml  pH meter End-over-end shaker SDS-PAGE buffers, reagents and equipment Optional: Western Blot reagents and equipment

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