PureCube Compact Cartridge Co-NTA

Order number: 31602

€52.00*

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Description

The PureCube Co-NTA Compact cartridge is an IMAC chromatography column prepacked with PureCube Co-NTA resin. You can choose between two sizes, 1 mL bed volume and 5 mL bed volume. The PureCube cartridge/column that is used here is compatible with almost all commonly used FPLC systems like ÄKTA TM.
If this is not the case we recommend buying the sole loose PureCube Co-NTA resin and packing the columns yourself. A dedicated tutorial can be found on the matching site.

Alternatively, we also offer our PureCube Co-NTA products as magnetic beads, if you prefer this IMAC method.
Feature
Usage Specific binding and purification of 6x His tagged proteins
Specificity Affinity to His tagged proteins
Binding capacity >30 mg/mL
Bead Ligand Co-NTA
Bead size 40 μm
Chelator stability Stable in buffer containing 10 mM DTT and 1 mM EDTA
Filling quantity Delivered as a 50 % suspension
Required equipment & materials
  • FPLC hardware like e.g. AEKTA
  • Binding Buffer
  • Elution Buffer
Feature - Column
PureCube Compact Cartridge 1 mL PureCube Compact Cartridge 5 mL
Bed Volume 1 mL 5 mL
Max. Flow Rate 6 mL/min 6 mL/min
Dimensions: diameter X length (mm) 5 X 35 17 X 35
Body material Polypropylene Polypropylene
Inlet 10-32 UNF female thread 10-32 UNF female thread
Outlet 10-32 UNF female thread 10-32 UNF female thread

Lab Results

Different metal ions confer different binding affinity and specificity

Loading different metal ions to a resin results in differing affinity and specificity for a His-tagged protein. Generally, cobalt exhibits the highest binding specificity of commonly used IMAC metal ions, leading to relatively low yields but high purity. Copper, at the other end of the spectrum, has a high affinity leading to high yields but unspecific binding. In searching for the optimal resin to purify a protein, it is recommended to explore different chelating ligands (IDA or NTA) and different metal ions.
Metal ions compared in their performance for His-tag purifications
Figure 1: Affinity and specificity of metal ions commonly used for IMAC. Loading an IMAC resin with different metal ions can adjust the affinity and specificity to optimize the purity and yield of a purified protein.

Video

Video Guide - FPLC

FAQ

Can I get the datasheet for the Co-NTA COMPACT cartridge?

Is there a difference between a column and a cartridge??

No, these two terms are synonymous.

What are the reasons for nonspecific binding?

Some histidine-rich proteins can also bind to cobalt, this is unavoidable. But washing with NaOH after elution of your protein of interest removes unspecific bound proteins from your resin.

Can Co-NTA beads be stripped?

Yes, they can. But we recommend using our pre-stripped NTA beads from the beginning:

Can I reuse PureCube Co-NTA beads or cartridges or can I regenerate them?

Yes, you can reuse the beads! We recommend using our washing & regeneration protocol at least after every 5th use of the beads.