We offer our PureCube Cobalt -NTA agarose resin pre-filled in Cartridges / Columns for FPLC based purification of His tagged proteins. The columns / cartridges are compatible with many FPLC machines like ÄKTAs. Refer to the corresponding features table to find out if they are matching your machinery. In case our columns do not match your machines try our COMPACT cartridges / columns . Addtionally we offer the same cartridges filled with smaller Co-NTA beads with a 40 µm diameter for higher flow rates.
Features - Regarding Purification capabilities.
Feature
Usage
Specific binding and purification of 6x his-tagged proteins
Specifity
Affinity to His-tagged proteins
Binding capacity
>30 mg/mL
Bead Ligand
Co-NTA
Bead size
30 μm
Matrix
7.5% highly cross-linked agarose
Chelator stability
Stable in buffer containing 10 mM DTT and 1 mM EDTA
ph Stability
2-14
Features - Regarding Cartridge / Column itself.
Feature
PureCube Compact Cartridge 1 mL
PureCube Compact Cartridge 5 mL
Bed Volume
1 mL
5 mL
Dimensions: diameter X length (mm)
6.2 x 50
11 x 80
Body material
Polypropylene
Acrylate
End Plug Material
Polypropylene
Inlet
10-32 UNF female thread
Outlet
10-32 UNF female thread
Max. Flow Rate
6 mL/min
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*The protocols on how to use the cartridges / columns are dependent on the used machine, therefore we unfortunately cannot provide a specific protocol here.
